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Product : Antibody >> Cancer Antibodies , Chromatin , DNA Methylation & Rep.. , Histone Deacetylases >> HDAC-11
      
 

Polyclonal Antibody to Histone Deacetylase 11 (HDAC-11)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-5027
HDAC-11Purified
0.1 mg
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Catalog No: IMG-5027
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein G Chromatography
Species: Human, Mouse, Rat
Host: Rabbit
Application: Western blot analysis: 1-3 ug/ml
Storage: Store antibody at 4°C for six months. For long term storage, store at -20°C.
Recommended
Positive Control:
human, mouse, or rat brain
 

Background
Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDACs are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). Recently, a new member of this family, HDAC-11, has been cloned by Gao et al (4). HDAC-11 transcripts were limited to kidney, heart, brain, skeletal muscle, and testis. These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Antigen
This antibody was developed by immunizing mice with a synthetic peptide (AHQGNGHERDFMDDKRVYI) of human HDAC-11.  This peptide sequence is 100% homologous in multiple HDAC11 isoforms. 

Application Notes
The peptide sequence used for immunogen is found in multiple HDAC-11 isoforms.  The observed molecular weight in western blots can vary depending on the isoform(s) detected. Users are encouraged to refer to NBCI data bases for more information about HDAC-11 isoforms and their amino acid size.    

Genebank Info (Protein): NP_055522
Gene ID: 9734
 
HDAC-11 Antibody

Western blot analysis of HDAC-11 using HDAC-11 antibody. Brain lysate from (A) human, (B) mouse and (C) rat brain probed with HDAC-11 antibody at 2 ug/ml.

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Reference
1. Gao L,  Cueto MA, Asselbergs F, and Atadja P. J. Biol. Chem. 28: 25748-25755 (2002).
2. Meinke PT and Liberator P. Curr Med Chem, 8(2):211-235 (2001).
3. Nakayama T and Takami Y. J Biochem (Tokyo) 2001, 129 (4):491-499 (20001).
4. Cress, W.D. and Seto, E. J. Cell. Physiol. 184, 1-16 (2000).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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