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Monoclonal Antibody to TLR4/CD284 (Clone 76B357.1) |
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| Catalog No: |
IMG-5031A |
| Contents: |
0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG2b, Kappa |
| Clone: |
76B357.1 |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse, Rat |
| Predicted React: |
Horse, Chimpanzee, Pig, Sheep, Bovine, Cat |
| Host: |
Mouse |
| Application: |
Flow (Cell Surface): use antibody at 0.5-2 ug/10^6 cells
Flow (Intracellular): See Cohen et al, 2008 for details.
Gel Shift:
IF: See Nowicki et al, 2009 for details.
IHC (cryosections): See Nowicki et al, 2012 for details.
IHC (paraffin): 5 ug/ml
Western blot analysis: 1-3 ug/ml |
| Storage: |
Store antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. |
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Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa (6). |
Antigen
This antibody was developed against a portion of amino acids 100-200 of human TLR4. |
Application Notes
The amino acid sequence used as immunogen for this antibdoy is 100% homologous in human, chimp, baboon, chinese hamster, and rat; 93% homologous in bovine and pig; 85% homologous in sheep and 78% homologous in cat, horse and mouse. |
Genebank Info (Protein)
NP_612564 |
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Western blot analysis of TLR4 antibody using TLR4 antibody at 2 ug/ml on (A) human intestine and 6 ug/ml on (B) mouse intestine and C) rat intestine lysate. |
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Flow analysis of cell surface TLR4 in mouse splenocytes using 0.5 ug/10^6 cells of antibody. Shaded histogram represents cells without anti-TLR4 antibody; green represents isotype control (BD Pharmingen) red represents anti-TLR4 antibody. |
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Formalin-fixed, paraffin-embedded human testis tissue stained with TLR4 antibody (5 ug/ml). |
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Reference
1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998).
2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001).
4. Takeuchi O., Hoshino K, Kawai T, Sanjo H, Takada H, Ogawa K, Takeda K and Akira S. Immunity 11: 443 (1999).
5. Poltorak A, Riccardi-Castagnoli P, Citterio S, and Butler B. Proc. Natl. Acad. Sci USA 97: 2163-2167 (2000).
6. Medzhitov R, Preston-Hurlburt P and Janeway CA Jr. Nature 388 (6640), 394-397 (1997).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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