PATHWAY PROFILING ANTIBODIES & REAGENTS

     
Products
Product : Antibody >> Antigen Affinity Purified , DNA Methylation & Rep.. , Neurobiology , New Antibodies , Phosphospecific Antibodie.. >> ATF2 (Ser490/498)
      
 

Polyclonal Antibody to ATF2 (Ser490/498)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-5040
ATF2 (Ser490/498)Purified, Peptide Affinity
0.1 mL
 --
 
Catalog No: IMG-5040
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 ml antibody in 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini Western Blots.
Isotype: Rabbit IgG
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Human
Host: Rabbit
Application: IHC (frozen):1:1000
Western blot analysis: 1:1000
Storage: For long-term storage 80C is recommended, but short-term storage at 20C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol.
Recommended
Positive Control:
human melanoma cells
 

Background
The activating transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF. Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. In addition, ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered important substrates of signals upstream of the activation of genes associated with neuronal growth and differentiation. ATF expression has also been linked to the depression in humans.

Antigen
A synthetic phosphopeptide corresponding to amino acids residues surrounding the human phospho Ser490/498 of ATF2 was used as immunogen. The antibody was purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns.

Application Notes
Specific for ~74 kDa ATF2 protein phosphorylated at Ser490/498. The antibody also recognizes the phosphorylated ~54 kDa splice form of ATF2. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state expected expression level.

Genebank Info (Protein): NP_001871
Gene ID: 1386
 
ATF2 (Ser490/498) Antibody

Immunolabeling of human melanoma cells incubated with varying doses of the radiomimetic drug NCS.

-

Reference
1. Karin, M. and Hunter, T. Transcriptional control by protein phosphorylation: signal transmissionfrom the cell surface to the nucleus, Curr. Biol. 5 (1995) 747 - 757.
2. Maekawa, T., Sakura, H., Kanei-Ishii, C., Sudo, T., Yoshimura, T. and Fujisawa, J. Leucinezipper structure of the protein CRE-BP1 binding to the cyclic AMP response element inbrain, EMBO J. 8 (1989) 2023 - 2028.
3. Laifenfeld, D., Karry, R., Grauer, E., Klein, E. and Ben-Shachar, D. ATF2, a member of theCREB/ATF family of transcription factors, in chronicstress and consequent toantidepressant treatment: animal models and human post-mortem brains, Neuropsychopharmacology 29 (2004) 589 - 97.
4. Ivanov, V.N., Bhoumik, A. and Ronai, Z. Death receptors and melanoma resistance toapoptosis, Oncogene 22 (2003) 3152 - 3161.
5. Gutpa, S., Campbell, D., Derijard, B. and Davis, R. J. Transcription factor ATF2 regulation by the JNK signal transduction pathway, Science 267 (1995) 389 - 393.
6. Bhoumik, A., Jones, N. and Ronai, Z. Transcriptional switch by activating transcription factor 2-derived peptide sensitizes melanoma cells to apoptosis and inhibits their tumorigenicity, Proc. Natl. Acad. Sci. U.S.A. 101 (2004) 4222 - 4227.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


Products

Services

Resources

Purchasing

Company

  • What's New
  • Reporter Cells
  • Screening Services
  • Interactive Pathways