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Polyclonal Antibody to Synaptotagmin (Ser309) |
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| Catalog No: |
IMG-5048 |
| Contents: |
0.1 ml antibody in 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini Western Blots. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 1:1000
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| Storage: |
For long-term storage –80°C is recommended, but short-term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. |
Recommended
Positive Control: |
rat brain |
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Background
Neurotransmitter release is mediated by exocytosis of synaptic vesicles at the presynaptic active zone of nerve terminals (1). Synaptic vesicles undergo a trafficking cycle to support rapid and repeated cycles of neurotransmitter release. Calcium-triggered exocytosis is the focal point of the vesicle cycle, which is followed by different routes of endocytosis and recycling. Recycling then leads to the docking and priming of the vesicles for another round of exocytocis and endocytosis.
The synaptotagmins are integral membrane proteins of synaptic vesicles. Synaptotagmins may serve as the calcium sensors that trigger neuortransmitter release (2,3). More than 13 synaptotagmins have been described, and synaptotagmin I is among the best characterized isoforms. Synaptotagmin I is an abundant constituent of synaptic vesicles that binds calcium ions through two C2 domains. It is thought that synaptotagmin I triggers exocytosis in a calcium dependent manner, and may also play a key role in endocytosis following membrane fusion. Synaptotagmin is phosphorylated by multiple protein kinases, and these phosphorylation events may play a regulatatory role in both exocytotic and endocytotic events. |
Antigen
A synthetic phosphopeptide corresponding to amino acids residues surrounding phospho Ser309 of synaptotagmin was used as immunogen. |
Application Notes
A ~60-62 kDa band is observed. Researchers are encouraged to optimize dilutions for their specific testing. This immunogen sequence is identical in synaptotagmins I, II and III in human, rat and mouse. |
| Genebank Info (Protein): NP_796376 |
| Gene ID: 127833 |
| SwissProt: Q8N9I0 |
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Western blot of 10 ug of rat brain lysate showing specific immunolabeling of synaptotagmin phosphorylated at Ser309 (Lane A). The labeling by the antibody was specifically blocked by the phosphopeptide used as antigen (Lane B). The corresponding non-phosphopeptide did not block the immunolabeling (not shown). |
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Reference
1. Sudhof TC. 2004. The synaptic vesicle cycle. Annu Rev Neurosci. 27:509-47.
2. Bai J and Chapman ER. 2004. The C2 domains of synaptotagmin--partners in exocytosis. Trends Biochem Sci. 3:143-51.
3. Chapman, E. 2002. Synaptotagmin: A Ca2+ sensor that triggers exocytosis. Nature Rev Mol Cell Biol. 3:498-508.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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