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Product : Antibody >> Antigen Affinity Purified , Neurobiology , New Antibodies , Stem Cell Markers >> Dopamine b-Hydroxylase (CT)
      
 

Polyclonal Antibody to Dopamine b-Hydroxylase (CT)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-5055
Dopamine b-Hydroxylase (CT)Purified, Peptide Affinity
0.1 mL
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Catalog No: IMG-5055
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 ml antibody in 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Isotype: Sheep Ig
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Bovine, Human, New World monkey, Rabbit
Host: Sheep
Application: Western blot analysis: 1:1000-1:2000
Storage: For long-term storage 80C is recommended, but short-term storage at 20C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol.
Recommended
Positive Control:
human adrenal medulla
 

Background
Dopamine beta-hydroxylase (DBH) catalyzes the conversion of dopamine to norepinephrine and serves as a marker of noradrenergic cells. Stress can result in the elevation of DBH.

Antigen
A synthetic peptide from the C-terminal region of human DBH was used as immunogen. The antibody was purified by affinity purification using a Sulfo-Link column matrix to which the peptide immunogen was coupled.

Application Notes
The antibody recognizes an epitope in the C-terminal region of dopamine beta-hydroxylase. Dopamine beta-hydroxylase is detected at ~75 kDa on western blots. Researchers are encouraged to optimize dilutions for their specific testing.

Genebank Info (Protein): NP_001029124
Gene ID: 796
 
Dopamine b-Hydroxylase (CT) Antibody

Western blot of human adrenal medulla. The antibody is specific for the ~75kDa DBH protein.

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Reference
1. Haycock, JW, Becker L, Ang L, Furukawa Y, Hornykiewicz O, and Kish SJ. 2003. Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum.  J. Neurochem. 87:574 - 585.
2. Waymire JC and Haycock  JW.  2002. Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells. J. Neurochem. 81:589 - 593.
3. Berry MD, Juorio AV, Li XM, and Boulton AA. 1996. Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme.  Neurochem. Res. 21:1075 -1087.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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