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Monoclonal Antibody to Sodium Calcium Exchanger 1 |
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| Catalog No: |
IMG-5068 |
| Contents: |
100 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS:137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. Adequate amount of material to conduct 10 mini Western Blots. |
| Isotype: |
Mouse IgG2b |
| Clone: |
6H2 |
| Purification: |
Protein G Chromatography |
| Species: |
Rat |
| Predicted React: |
Most Mammals |
| Host: |
Mouse |
| Application: |
Western blot analysis: 1:1000-1:2000 |
| Storage: |
The lyophilized antibody can be stored at -20°C and is stable for at least 2 years. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS:137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody should be aliquot and stored at -20°C. |
Recommended
Positive Control: |
rat brain, heart, kidney, lung, spleen, liver, or skeletal muscle |
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Background
Ca2+ plays a critical role in intracellular signaling and thus regulation of calcium balance is critical for the maintenance of this signaling function. The plasma membrane Na+/Ca2+ (NaCX) exchangers mediate Na+-dependent Ca2+ efflux in a wide variety of cell types (reviewed in 1-3). NaCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). NCX1 is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling. |
Antigen
A fusion protein from the N-terminus of the rabbit renal sodium calcium exchanger was used as immunogen. The antibody was purified by Protein G affinity chromatagraphy. |
Application Notes
Specific for the ~120 kDa and ~160k Na+/Ca2+ Exchanger NaCX-1 protein in Western Blots with broad tissue and species specificities. Antibody dilutions and tissue load should be based on tissue type and expected expression level. |
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Western blot of various rat tissue lysates showing immunolabeling of the ~120 kDa NCX1 protein. Brain (Lane A), Heart (Lane B), Kidney (Lane C), Lung (Lane D), Spleen (Lane E), Liver (Lane F), Skeletal muscle (Lane G). |
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Reference
1. Hilgemann DW.2004. New insights into the molecular and cellular workings of the cardiac Na+/Ca2+ exchanger.Am J Physiol Cell Physiol. 287:C1167-C1172.
2. Canitano A, Papa M, Boscia F, Castaldo P, Sellitti S, Taglialatela M, Annunziato L. 2002. Brain distribution of the Na+/Ca2+ exchanger-encoding genes NCX1, NCX2, and NCX3 and their related proteins in the central nervous system.Ann N Y Acad Sci. 976:394-404.
3. Quednau BD, Nicoll DA, Philipson KD. 2004. The sodium/calcium exchanger family-SLC8. Pflugers Arch. 447:543-548.
4. Li XF, Lytton J. 1999. A cicularized sodium-calcium exchanger exon 2 transcript. J Biol Chem. 274:8153-8160.
5. Szerencsei RT, Tucker JE, Cooper CB, Winkfein RJ, Farrell PJ, Iatrou K, Schnetkamp PPM. 2000. Minimal domain requirement for cation transport by the potassium-dependent Na/Ca-K exchanger. 275:669-676.
6. Grosshans DR, Clayton DA, Coultrap SJ, Browning MD. 2002. LTP leads to rapid surface expression on NMDA not AMPA receptors in adult rat CA1. Nature Neurosci. 5:27 - 33.
7. Luo JH, Wang YH, Yasuda RP, Dunah AW, Wolfe BB. 1997. The majority of N-methyl-D-aspartate receptor complexes in adult rat cerebral cortex contain at least three different subunits (NR1/NR2A/NR2B). Mol. Pharmacol. 51:79 - 86.
8. Zukin RS and Bennett MVL. 1995. Alternatively spliced isoforms of the NMDARI receptor subunit. Trends Neurosci., 18:306 - 313.
9. Laurie DJ. and Seeburg PH. 1994. Regional and developmental heterogeneity in splicing of the rat brain NMDAR1 mRNA. J. Neurosci. 14:3180 - 3194.
10. Foldes RL, Rampersad V, Kamboj RK. 1994. Cloning and sequence analysis of additional splice variants encoding human N-methyl-D-aspartate receptor (hNR1) subunits. Gene 147:303 - 304.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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