Polyclonal Antibody to RANKL (CD254)

Background
CD254 is a member of the tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells (1-2). CD254 (RANKL) binds to RANK on DCs, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). CD254 is also important in Tcell and Bcell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), CD254 can both activate mature osteoclasts and mediate osteoclastogenesis (4,5). CD254 deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3). Mouse CD254 is a 316 amino acid protein.

Antigen
A synthetic peptide corresponding to amino acids 161-177 of mouse CD254 (AQPFAHLTINAASIPSG) was used as immunogen for this antibody.

Application Notes
The CD254 amino acid sequence used as immunogen for this antibody is 88% homologous in human and 94% homologous in rat.

Genebank Info (Protein)
NP_003692

Reference
1. Hsu, H., Lacey, D.L., Dunstan, C.R., Solovyev, I., et al. Proc. Natl. Acad. Sci. USA 96: 3540-3545 (1999).
2. Lacey, D.L. Timms, E,. Tan, H.L., Kelly, M.J. , et al. Cell 93: 165-176 (1998).
3. Kong, Y-Y., Yoshida, H., Sarosi, I., Tan, H-L., et al. Nature 397: 315-323 (1999).
4. Wong, B.R., Josien, R., Lee, S.W., Sauter, B., Li, H-L., Steinman, R.M., and Choi, Y. J. Exp. Med. 186: 2075-2080.
5. Anderson, D.M, Maraskovsky, E., Billingsley, W.L., Dougall, W.C., Tometsko, M.E., Roux, E.R., Teepe, M.C., DuBose, R.F., Cosman, D., and Gaibert, L. Nature 390: 175-179 (1997).
6. Y. Wittrant, Y. Gorin, S. Mohan, B. Wagner, S. L. Abboud-Werner.  Colony-Stimulating Factor-1 (CSF-1) Directly Inhibits Receptor Activator of Nuclear Factor-κB Ligand (RANKL) Expression by Osteoblasts.  Endocrinology. 2009 November; 150(11): 4977–4988.
7. Eun Young Lee, MiRan Seo, Yong-Sung Juhnn, Jeong Yeon Kim, Yoo Jin Hong, Yun Jong Lee, Eun Bong Lee, Yeong Wook Song.  Potential role and mechanism of IFN-gamma inducible protein-10 on receptor activator of nuclear factor kappa-B ligand (RANKL) expression in rheumatoid arthritis.  Arthritis Res Ther. 2011; 13(3): R104.
8. Mehdi Shakibaei, Constanze Buhrmann, Ali Mobasheri.  Resveratrol-mediated SIRT-1 Interactions with p300 Modulate Receptor Activator of NF-κB Ligand (RANKL) Activation of NF-κB Signaling and Inhibit Osteoclastogenesis in Bone-derived Cells.  J Biol Chem. 2011 April 1; 286(13): 11492–11505.
9. Wendy Tseng, Lucia S. Graham, Yifan Geng, Aneela Reddy, Jinxiu Lu, Rita B. Effros, Linda Demer, Yin Tintut.  PKA-induced Receptor Activator of NF-κB Ligand (RANKL) Expression in Vascular Cells Mediates Osteoclastogenesis but Not Matrix Calcification.  J Biol Chem. 2010 September 24; 285(39): 29925–29931.