Polyclonal Antibody to RANK/TNF11aR/ODFR

Background
Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand  (RANKL/TRANCE/ OPGL) (mAbs to RANKL is also available from Imgenex, Cat # IMG-133) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-X_L suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathway. Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2.

Antigen
A synthetic peptide corresponding to amino acid residues 603-613 of human RANK. Genbank accession number: NP_003830.               

Genebank Info (Protein)
NP_003830

Reference
1. Anderson,D.M., Maraskovsky,E., Billingsley,W.L., Dougall,W.C., et al. A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function. Nature 390 (6656), 175-179 (1997).
2. Darnay,B.G., Ni,J., Moore,P.A. and Aggarwal,B.B. Activation of NF-kappaB by RANK requires tumor necrosis factor receptor-associated factor (TRAF) 6 and NF-kappaB-inducing kinase. Identification of a novel TRAF6 interaction motif. J. Biol. Chem. 274 (12), 7724-7731 (1999).
3. Hsu,H., Lacey,D.L., Dunstan,C.R., Solovyev,I., Colombero,A., et al. Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand. Proc. Natl. Acad. Sci. U.S.A. 96 (7), 3540-3545 (1999).
4. Dougall,W.C., Glaccum,M., Charrier,K., Rohrbach,K., Brasel,K., De Smedt,T.,  et al. RANK is essential for osteoclast and lymph node development. Genes Dev. 13 (18), 2412-2424 (1999).
5. Karsdal,M.A., Hjorth,P., Henriksen,K., Kirkegaard,T., Nielsen,K.L., et al. Transforming growth factor-beta controls human osteoclastogenesis through the p38 MAPK and regulation of RANK expression. J. Biol. Chem. 278 (45), 44975-44987 (2003).