Polyclonal Antibody to Lamin B1

Background
The lamins form a meshwork of filaments on the inner, or nucleoplasmic, surface of the nuclear envelope. Currently, mammalian species appear to contain at least four major lamins, called A, B1, B2, and C, of which two (A and C) arise by alternative splicing of the same gene and two minor lamins, D and E, whose molecular weight varies from species to species. Nuclei of embryonic and lymphiod cells contain only lamin B1. Mutations affecting A-type lamins have been associated with a variety of human diseases, including muscular dystrophy, cardiomyopathy, lipodystrophy, and progeria, but mutations in B-type lamins have never been identified in humans.  Homozygous lamin B1 mutant mice survive embryonic development but died at birth with defects in lung and bone. Cells obtained from mutant lamin B1 mice are defective in differentiation, polyploidy and senesce prematurely.

Antigen
Full length recombinant Lamin B1 protein was used as the immunogen.

Application Notes

Reference
1. Laurent Vergnes,  Miklós Péterfy,  Martin O. Bergo,  Stephen G. Young, and Karen Reue. Lamin B1 is required for mouse development and nuclear integrity.  Proc Natl Acad Sci U S A. 101(28): 10428–10433 (2004).
2. Spyros D. Georgatos. The inner nuclear membrane: simple, or very complex? EMBO J.  20(12): 2989–2994 (2001).
3. Peter Hornbeck, Kuo-Ping Huang, and William E. Paul. Lamin B is rapidly phosphorylated in lymphocytes after activation of protein kinase C. Proc. Nall. Acad. Sci. USA  85: 2279-2283 (1998).