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Product : Antibody >> Neurobiology , New Antibodies , Nuclear & Cytoplasmic.. >> Lamin B1
      
 

Polyclonal Antibody to Lamin B1

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-5133A
Lamin B1Purified
0.1 mg
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Catalog No: IMG-5133A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ugs in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Clone: N/A
Purification: Protein G Chromatography
Species: Human
Host: Rabbit
Application: Western blot analysis: 0.5-2 ug/ml
Storage: Store at 4°C, stable for 6 months. For long-term storage, store at -20°C.
Recommended
Positive Control:
HeLa or Hep-2 whole cell lysate  
 

Background
The lamins form a meshwork of filaments on the inner, or nucleoplasmic, surface of the nuclear envelope. Currently, mammalian species appear to contain at least four major lamins, called A, B1, B2, and C, of which two (A and C) arise by alternative splicing of the same gene and two minor lamins, D and E, whose molecular weight varies from species to species. Nuclei of embryonic and lymphiod cells contain only lamin B1. Mutations affecting A-type lamins have been associated with a variety of human diseases, including muscular dystrophy, cardiomyopathy, lipodystrophy, and progeria, but mutations in B-type lamins have never been identified in humans.  Homozygous lamin B1 mutant mice survive embryonic development but died at birth with defects in lung and bone. Cells obtained from mutant lamin B1 mice are defective in differentiation, polyploidy and senesce prematurely.

Antigen
Full length recombinant Lamin B1 protein was used as the immunogen.

Application Notes

 

 
Lamin B1 Antibody

Western blot analysis of Lamin B1 in 30 ugs of A) HeLa and B) Hep-2 whole cell lysate using IMG-5133A at 0.5 ug/ml.

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Reference
1. Laurent Vergnes,  Miklós Péterfy,  Martin O. Bergo,  Stephen G. Young, and Karen Reue. Lamin B1 is required for mouse development and nuclear integrity.  Proc Natl Acad Sci U S A. 101(28): 10428–10433 (2004).
2. Spyros D. Georgatos. The inner nuclear membrane: simple, or very complex? EMBO J.  20(12): 2989–2994 (2001).
3. Peter Hornbeck, Kuo-Ping Huang, and William E. Paul. Lamin B is rapidly phosphorylated in lymphocytes after activation of protein kinase C. Proc. Nall. Acad. Sci. USA  85: 2279-2283 (1998).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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