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Datasheet
Polyclonal Antibody to beta Actin
Cat.No IMG-5142A
Description b-actin - Loading Control
Unit 0.1 mg
Price(USD) 225.00
MSDS
Catalog No
:
IMG-5142A
Contents
:
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype
:
Rabbit IgG
Clone
:
N/A
Purification
:
Protein G chromatography
Species
:
Cow, Human, Mouse, Rat
Predicted React
:
Dog, Pig, Rabbit, Sheep
Host
:
Rabbit
Application
Western blot analysis: 0.25-1 ug/ml Storage Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. Recommended Positive Control : human brain, lung, spleen
Antigen
Amino acids 2-16 (CDDDIAALVIDNGSG) of actin protein were used as the immunogen.
Application Notes
The NCBI accession number for actin is AAA51567. This antibody can not be used to test heart or muscle lysate loading.
Western blot analysis of beta actin in A: human brain (40141), B: mouse brain (40121), C: rat brain (40121), D: human lung (40146) and E: human spleen (40149) tissue lysates using IMG-5142A at 0.25 ug/ml.
Western blot analysis of beta actin in decreasing amounts of human ovary tissue lysate using IMG-5142A at 0.25 ug/ml: A) 40 ug, B) 30 ug, C) 20 ug, and D) 10 ug per lane.
Product Citations 1. Gfra1 silencing in mouse spermatogonial stem cells results in their differentiation via the inactivation of RET tyrosince kinase. He Z, M-C Hofmann, and M Dym. Biology of Reproduction 77: 723-733 (2007). Imgenex antibodies cited for WB (mouse sperm): 1. GFRA1 (IMG-192), Figs 1B, 5D (siRNA validated). 2. Rabbit IgG-HRP (20103), Figs 1B, 5D. 3. Beta-actin (IMG-5142A), Figs 1B, 5D, 8B&E. 2. GDNF up-regulates c-fos transcription bia the Ras/ERK1/2 pathway to promote mouse spermatogonial stem cell proliferation. He Z, J Jiang, M Kokkinaki, N Golestaneh, M-C Hofmann, M Dym. Stem Cells DOI: 10.1634/stemcells.2007-0436. WB (mouse C18-4 cell line): Figs. 1Y, 4A. 3. Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain. Gerstner J, B Quentin, W Heyden, T Lavaute, J Yin, C Landry. PLoS ONE 3: e1631 (2008). Imgenex antibodies cited [WB (mouse brain tissue)]: 1. GAPDH - Loading Control (IMG-3073): Figs. 1A,B; 3. 2. b-actin (IMG-5142A): Fig. 3.
4. Spinal antiallodynia action of glycine transporter inhibitors in neuropathic pain models in mice. Morita K, N Motoyama, T Kitayama, N Morioka, K Kifune, T Dohi. Behavioral Pharmacology 326: 633-645 (2008). WB (mouse spinal cord tissue lysates), Fig. 2A,B. 5. Osteopontin: a novel cytokine involved in the regulation of Mycobacterium avium subspecies paratuberculosis infection in periparturient dairy cattle. Karcher E, D Byles, J Bannatine, D Beitz, J Stabel. J Dairy Science 91: 3079-3091 (2008). WB (cow PBMCs), Fig. 9A. 6. HTLV-1 tax is a critical lipid raft modulator that hijacks IkB kinases to the microdomains for persistant activation of NF-kB. Huang J, T Ren, H Guan, Y Jiang, H Cheng. JBC 10.1074/jbc.M806390200 (2009). WB (human T cell lines), Fig. 1B.
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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The protocols described here can be used as a guide line for use of IMGENEX Histo-array slides. Modifications may be necessary depending on the target gene(s), antibodies, or nucleic acid probes.
