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Polyclonal Antibody to GAPDH |
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| Catalog No: |
IMG-5143A |
| Contents: |
GAPDH antibody at 0.5 mg/ml in PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Protein G chromatography |
| Species: |
Cat, Chimpanzee, Drosophila, Human, Macaque, Mouse, Pig, Rat |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 0.25-1.0 ug/ml |
| Storage: |
Store GAPDH antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C. |
Recommended
Positive Control: |
multiple cell lines and tissues including HeLa and Lung |
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Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots. |
Antigen
Amino acids 73-87 (PITIFQERDPSKIKW) of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen for this antibody. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, baboon, chimp and macaque, 93% in pig, rat and gerbil, and 86% mouse, cat and salamander. |
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Western blot analysis of GAPDH in the multiple human tumor cell line lysate INSTA-Blot (IMB-105) using GAPDH antibody at 0.25 ug/ml. |
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Western blot analysis of GAPDH using GAPDH antibody at 1:500 in nuclear and cytoplasmic fractions made from Drosophila head extracts. Data courtesy of Dr. Jerry Lin, University of Wisconsin-Madison. |
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Reference
1. Burke JR, et al. Nat Med. 1996 Mar;2(3):347-50. PMID: 8612237
2. Fortun, J. et al. J. Neurosci. 23: 10672-10680.
3. Ellis,R.C. et al. J. Nuerochem. 88:689-697.
4. Nilkantha Sen, Makoto R. Hara, Michael D. Kornberg, Matthew B. Cascio, Byoung-Il Bae, Neelam Shahani, Bobby Thomas, Ted M. Dawson, Valina L. Dawson, Solomon H. Snyder, Akira Sawa. Nitric oxide-induced nuclear GAPDH activates p300/CBP and mediates apoptosis. Nat Cell Biol. 2008 July; 10(7): 866–873.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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