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Polyclonal Antibody to SUMO3 (SMT3) |
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| Catalog No: |
IMG-5196A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A chromatography |
| Species: |
Chimpanzee, Horse, Human, Mouse, New World monkey |
| Predicted React: |
Opossum, Pufferfish |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 2-4 ug/ml
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| Storage: |
Store SUMO3 (SMT3) antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C. |
Recommended
Positive Control: |
Testis |
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Background
SUMO3 (SMT3) is a Ubiquitin-like protein found in Saccharomyces cerevisiae similar to the mammalian Ubiquitin-like SUMO1, which is involved in posttranslational protein modification. SUMO3 is functionally active in regulation of nucleocytoplasmic transport and suggest a role in cell-cycle regulation. Knockdown of SUMO3 in Drosophila in the prothoracic gland, which controls key developmental processes through the synthesis and release of ecdysteroids, caused a 4-fold prolongation of larval life and completely blocked the transition from larval to pupal stages. |
Antigen
A portion of amino acids 50-95 of human SUMO3 (SMT3) was used as the immunogen for this antibody. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, horse, rhesus monkey, mouse and rat, 90% homologous in pufferfish and 81% homologous in opossum. |
| Genebank Info (Protein): NP_579932 |
| Gene ID: 170930 |
| SwissProt: P61957 |
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Western blot analysis of SUMO3 (SMT3) in human testis lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse testis lysate using SUMO3 antibody at 2 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution (10087K) were used for this test. |
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Reference
1. Talamillo, A. et al. Development 135: 1659-1668 (2008).
2. Johnson, E.S. et al. Embo. J. 16: 5509-5519 (1997).
3. Wanyun, S. et al. Protein Sci. 11: 1482-1491 (2002).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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