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Product : Antibody >> New Antibodies , Nuclear & Cytoplasmic.. , Transcription Factors >> BiP/GRP78
      
 

Polyclonal Antibody to BiP/GRP78

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-5228A
BiP/GRP78 Purified
0.1 mg
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Catalog No: IMG-5228A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ugs in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Clone: N/A
Purification: Protein G Chromatography
Species: Human, Mouse
Predicted React: Chimpanzee, Dog, Macaque, Rat, Xenopus, Zebrafish
Host: Rabbit
Application: IF/ICC: See Egawa et al (2011).
IHC (paraffin): 5 ug/ml
Western blot analysis: 1-3 ug/ml
Storage: Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C.
 

Background
GRP78, a 78 kDa glucose-regulated protein, also known as BiP or immunoglobulin heavy chain binding protein, is a stress-response protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function (2). This protein is essential for the proper glycosylation, folding and assembly of many membrane bound and secreted proteins (3). GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis (4). GRP78 protein levels have been shown to be a reliable biomarker of hypoglycermia (5) as well as serving a neuroprotective function in neurons exposed to glutamate and oxidative stress (2). GRP78 levels are reduced in the brains of Alzheimer's Disease patients and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene (6). The induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs (7).

Antigen
A portion of amino acids 550-600 of human GRP78 was used as the immunogen.

Application Notes
The NCBI accession number for BiP/GRP78 is AAF42836.  The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, dog, and chimp.

Genebank Info (Protein): AAF42836
Gene ID: 3309
SwissProt: P11021
 
BiP/GRP78 Antibody

Western blot analysis of GRP78 in A) HeLa, B) human liver, C) mouse liver, and D) rat liver lysate using IMG-5228A at 2 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.

BiP/GRP78 Antibody IMG-5228A

Western blot analysis of GRP78 in mouse liver tissue enriched using IMGENEX's Endoplasmic Reticulum Enrichment Kit (IMGENEX, 10088K) and 1 ug/ml of IMG-5228A (anti-GRP78) and 0.25 ug/ml of IMG-5143A (anti-GAPDH loading control). Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction.  IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.

BiP/GRP78

Immunohistochemical analysis of GRP78 in formalin-fixed, paraffin-embedded mouse liver tissue using IMG-5228A at 5 ug/ml.

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Reference
1. Corrigall,V.M., Bodman-Smith,M.D., Brunst,M., Cornell,H. and Panayi,G.S., Inhibition of antigen-presenting cell function and stimulation of human peripheral blood mononuclear cells to express an antiinflammatory cytokine profile by the stress protein BiP: relevance to the treatment of inflammatory arthritis, Arthritis Rheum. 50 (4), 1164-1171 (2004).
2. Reddy,R.K., Mao,C., Baumeister,P., Austin,R.C., Kaufman,R.J. and Lee,A.S., Endoplasmic reticulum chaperone protein GRP78 protects cells from apoptosis induced by topoisomerase inhibitors: role of ATP binding site in suppression of caspase-7 activation, J. Biol. Chem. 278 (23), 20915-20924 (2003).
3. Watson,L.M., Chan,A.K., Berry,L.R., Li,J., Sood,S.K., Dickhout,J.G., Xu,L., Werstuck,G.H., Bajzar,L., Klamut,H.J. and Austin,R.C., Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity J. Biol. Chem. 278 (19), 17438-17447 (2003).
4. Shuda,M., Kondoh,N., Imazeki,N., Tanaka,K., Okada,T., Mori,K., Hada,A., Arai,M., Wakatsuki,T., Matsubara,O., Yamamoto,N. and Yamamoto,M., Activation of the ATF6, XBP1 and grp78 genes in human hepatocellular carcinoma: a possible involvement of the ER stress pathway in hepatocarcinogenesis, J. Hepatol. 38 (5), 605-614 (2003).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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