Datasheet

Polyclonal Antibody to IkBa/IkBa Cat.No IMG-5270A Description IkBa Format Purified Unit 0.1 mg Price(USD) 205.00 MSDS Catalog No : IMG-5270A Contents : 100 ug in 200 ul PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. Isotype : Rabbit IgG Clone : N/A Purification : Protein G Chromatography Species : Human Host : Rabbit Application Western blot analysis: 0.5-2 ug/ml Storage Store at 4°C. For long-term storage, store at -20°C. Recommended Positive Control : Daudi Background NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa. Antigen A full-length recombinant protein of human IkBa was used as immunogen. Application Notes This specific epitope of this antibody has not been mapped, but it does not appear to detect  mouse IkBa. Genebank Info (Protein) NP_065390   Western blot analysis of IkBa in A) Daudi and B) 3T3 using IMG-5270A at 1 µg/mL.  No cross-reacting is seen with the mouse cell line. ffsdf Related Products 1. 20301 [Goat Anti-Rabbit HRP Conjugate] 2. 40163 [Daudi cell line lysate (lymphoma, Burkitt)] Reference 1. Verma, I.M., Genes. Dev. 9: 2723-2735, 1995. 2. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci USA 94: 11758 (1997). 3. DiDonato, J.A., et al. Nature 388: 548- (1997). 4. Regnier, C.H., et al. Cell 90, 373 (1997). 5. Mercurio, F., et al. Science 278: 860 (1997). 6. Yamaoka, S., et al. Cell 93: 1231-1240 (1998). 7. Sanjo H, Kawai T, and Akira S. DRAKs, novel serine/threonine kinases related to death associated protein kinase that trigger apoptosis. J Biol Chem 273 (44): 29066-29071, 1998. 8. Matsumoto M, Takeda K, Sanjo H, and Akira S. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol 18 (3): 1642-1651, 1998. Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.

Related Products

Related Product(s) of the Catalog # IMG-5270A

Cat. No Description Format Species Appl. Qty Price(USD) 20301 Anti-Rabbit Ig HRP Conjugate HRP R ELISA, WB 0.1 mL 80.00 Cat. No Description Species Qty Price(USD) 40163 Daudi cell line lysate (lymphoma, Burkitt) H 0.1 mg 84.00

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Protocols

Imgenex Protocols
1.	Histo-Array Protocols
The protocols described here can be used as a guideline for use of IMGENEX Histo-array slides. Modifications may be necessary depending on the target gene(s), antibodies, or nucleic acid probes.
2.	INSTA-Blot Protocol
Western Blot protocol for INSTA-Blot Ready-to-Use membrane
3.	Custom Polyclonal Antibody Production Protocol
Custom Polyclonal Antibody Production Protocol
4.	IMGENEX Western Blot Protocol
IMGENEX Western Blot protocol
5.	Intracellular Staining Flow Protocol
Catalog No. 10083K
6.	Western Blot Protocol for Goat Antibodies
Western Blot Protocol for Goat Antibodies
7.	Tissue Lysate Preparation Protocol
Imgenex Tissue Lysate Preparation Protocol
8.	Cell Lysate Preparation Protocol
Imgenex Cell Lysate Preparation Protocol
9.	DNA Isolation
DNA Isolation
10.	IHC Protocol for Antibody Detection in Formalin fixed Tissue
IHC Protocol for Antibody Detection in Formalin fixed Paraffin Embedded Tissues
11.	Cell Surface Staining Flow Protocol
Catalog No. 10084K
12.	Immunohistochemistry Protocol for IMA Microarray Slides
The protocols described here can be used as a guideline for use of IMGENEX Histo-array slides. Modifications may be necessary depending on the target gene(s), antibodies, or nucleic acid probes.

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