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Datasheet

 
Polyclonal Antibody to Anterior Gradient 2 (AGR2) Homolog
Cat.No
IMG-5279A
Description
Anterior Gradient 2 Homolog (AGR2)
Format
Purified
Unit
0.1 mg
Price(USD)
215.00
MSDS
View MSDS
Catalog No : IMG-5279A
Contents : 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype : Rabbit IgG
Clone : N/A         
Purification : Protein G Chromatography
Species : Human, Mouse
Predicted React : Rat, Chimpanzee, Dog, Bovine, Xenopus, Chicken, Orangutan
Host : Rabbit
Application

IHC (frozen): See Park et. all (2009) for details.
IHC (paraffin): 1:100
IP: See Park et. all (2009) for details.
Western blot analysis: 1:100
Storage
Store at 4 deg C, stable for 6 months. For long-term storage, store at -20 deg C.

Background
hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR-1/2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development(1). Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.

Antigen
This antibody was developed against synthetic peptides corresponding to amino acids 55-72 (TQTYEEALYKSKTSNKPL) of human AGR2; Genbank accession no. NP_006399.1

Application Notes
Researchers are encouraged to use the sequence and accession number (NP_006399.1) that were used to design the peptide immunogen in order to assess potential cross-reactivity of the antibody to their species of interest.

Genebank Info (Protein)
NP_006399

 

Immunohistochemical staining with IMG-5279A, anti-AGR2 antibody, in formalin-fixed, paraffin-embedded normal colon tissue at 1:100 dilution.

Western blot analysis of AGR2 in A) MCF7, B) Caco-2 and C) human stomach lysate using IMG-5279A at 1:100. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. The higher molecular weight band of variable intensity at ~30 kDa is uncharacterized and may represent a form of AGR2.

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Reference
1. Fletcher GC et al.,Br J Cancer. 2003 Feb 24;88(4):579-85.
2. Petek E et al.,Cytogenet Cell Genet. 2000;89(3-4):141-2.


Product Citations
1. Proteomic changes during intestinal cell maturation in vivo.  Chang J, MR Chance, C Nicholas, N Ahmed, S Guilmeau, M Flandez, D Wang, D-S Byun, S Nasser, JM Albanese, GA Corner, BG Heerdt, AJ Wilson, LH Augenlicht, JM Mariadason.  J Proteomics 71:530-546 (2008). IHC (paraffin): mouse intestine tissue, Fig 5.
2. The adenocarcinoma-associated antigen, AGR2, promotes tumor growth, cell migration, and cellular transformation.  Wang Z, Y Hao, A Lowe. Cancer Research 68: 492-497 (2008). IHC (paraffin): mouse intestine tissue, Fig 3C.
3. SPDEF is required for mouse pulmonary goblet cell differentiation and regulates a network of genes associated with mucus production. Chen G, TR Korfhagen, Y Xu, J Kitzmiller, SE Wert, Y Maeda, A Gregorieff, H Clevers, JA Whitsett.  J Ciin Invest 119:2914-2924 (2009). IHC (paraffin): transgenic mouse lung tissue, Fig 4B, C.
4. Analysis of the pancreatic tumor progression by a quantitative proteomic approach and immunhistochemical validation. Sitek B, B Sipos, I Alkatout, G Poschmann, C Stephan, T Schulenborg, K Marcus, J Luttges, D Dittert, G Baretton, W Schmiegel, S Hahn, G Kloppel, H Meyer, K Stuhler. J Proteo Res 8: 1647-1656 (2009). IHC (paraffin): Normal human epithelium, PanIN (pancreatic intraepithelial neoplasia) lesions, carcinoma, and pancreatis, Fig 2.
5. The protein disulfide isomerase AGR2 is essential for production of intestinal mucus. Park S, G Zhen, C Verhaeghe, Y Nakagami, L Nguyenvu, A Barczak, N Killeen, D Erle. PNAS 106: 6950-6955 (2009).
1. IHC (frozen): Mouse intestinal villi and colon mucous granules, Fig 1 & B. 
2. IHC (paraffin):  The specifity of the antibody was confirmed by IHC (p) in AGR2 wildtype and knock out mice. Staining was seen in AGR2 +/+ but not AGR2 -/- mice, Fig 2A-2D.
3. IHC (paraffin): Small intestine from AGR2 -/- mice, Fig S4B.
4. IP: LS174T (human colon tumor) cell line;  AGR2 pAb CO-IP'd MUC2. 


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.

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IMG-5279A [Polyclonal Antibody to Anterior Gradient 2 (AGR2) Homolog]
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