Background
hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR-1/2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development(1). Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.

Antigen
This antibody was developed against synthetic peptides corresponding to amino acids 55-72 (TQTYEEALYKSKTSNKPL) of human AGR2; Genbank accession no. NP_006399.1

Application Notes
Researchers are encouraged to use the sequence and accession number (NP_006399.1) that were used to design the peptide immunogen in order to assess potential cross-reactivity of the antibody to their species of interest.

Genebank Info (Protein)
NP_006399

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Reference
1. Fletcher GC et al.,Br J Cancer. 2003 Feb 24;88(4):579-85.
2. Petek E et al.,Cytogenet Cell Genet. 2000;89(3-4):141-2.

Product Citations
1. Proteomic changes during intestinal cell maturation in vivo.  Chang J, MR Chance, C Nicholas, N Ahmed, S Guilmeau, M Flandez, D Wang, D-S Byun, S Nasser, JM Albanese, GA Corner, BG Heerdt, AJ Wilson, LH Augenlicht, JM Mariadason.  J Proteomics 71:530-546 (2008). IHC (paraffin): mouse intestine tissue, Fig 5.
2. The adenocarcinoma-associated antigen, AGR2, promotes tumor growth, cell migration, and cellular transformation.  Wang Z, Y Hao, A Lowe. Cancer Research 68: 492-497 (2008). IHC (paraffin): mouse intestine tissue, Fig 3C.
3. SPDEF is required for mouse pulmonary goblet cell differentiation and regulates a network of genes associated with mucus production. Chen G, TR Korfhagen, Y Xu, J Kitzmiller, SE Wert, Y Maeda, A Gregorieff, H Clevers, JA Whitsett.  J Ciin Invest 119:2914-2924 (2009). IHC (paraffin): transgenic mouse lung tissue, Fig 4B, C.