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Datasheet
Polyclonal Antibody to TLR3/CD283 (Phospho Tyr759)
Cat.No IMG-5348A
Description TLR3/CD283 (Tyr759)
Unit 0.02 mg
Price(USD) 330.00
MSDS
Catalog No
:
IMG-5348A
Contents
:
20 ug in 100 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype
:
Rabbit Ig
Purification
:
Antigen Affinity Chromatography
Species
:
Human
Predicted React
:
Bovine, Chimpanzee, Dog, Monkey, Mouse, Rat
Host
:
Rabbit
Application
IF/ICC: see W. Xu. et. al (2008) for details IHC (frozen): see W. Xu. et. al (2008) for details Western blot analysis: 0.1-1.0 ug/ml Storage Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C.
Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). TLR3 cDNA codes for a protein with approximate molecular weight of 120 kDa (4). TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-6). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes (6). The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.
Antigen
A portion of amino acids 750-800, containing a phosphorylated tyrosine residue at position 759, of human TLR3 was used as the immunogen.
Western blot analysis of phosphorylated TLR3 in (A) recombinant fusion protein containing a phosphorylated tyrosine at position 759 and (B) fusion protein containing an unphosphorylated tyrosine at position 759, using IMG-5348A at 0.1 ug/ml.
Immunofluorescence analysis of IgG, AID and TLR3 (IMG-5348) expression in a healthy donor (A) and a hyper-IgM (HIGM) syndrome patient (B and C). A and B: Paraformaldehyde-fixed, frozen tonsillar mucosa tissue sections. C. Paraformaldehyde-fixed, mucosal B cells from the colon lamina propria of the HIGM patient. Arrowheads indicate cells co-expressing phospho TLR3, AID, and IgG. Data courtesy of W. Xu. et. al., and the Journal of Immunology
Reference 1. Muzio M, Bosisio D, Polentarutti N, D'amico G, Stoppacciaro A, Mancinelli R, van't Veer C, Penton-Rol G, Ruco LP, Allavena P, Mantovani A. J Immunol 164(11):5998-6004 (2000). 2. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998). 3. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 4. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001). 5. Rock FL., Hardiman G., Timans JC., Kastelein RA. and Bazan JF. Proc. Natl. Acad. Sci. U.S.A. 95 (2), 588-593 (1998). 6. Muzio M, Polentarutti N, Bosisio D, Prahladan MK, Mantovani A. J Leukoc Biol 67(4):450-456 (2000). 7. Sarkar SN, Peters KL, Elco CP, Sakamoto S, Pal S& Sen GC. Nature Structural & Molecular Biology, 11, 1060-1067 (2004).
Product Citations 1. Viral double-stranded RNA triggers Ig class switching by activating upper respiratory mucosa B cells through an innate TLR3 pathway involving BAFF. Xu W, PA Santini, AJ Matthews, A Chiu, A Plebani, B He, K Chen and A Cerutti. J Immunol 181:276-287 (2008). IHC (F):Fig 4A and B, Paraformaldehyde-fixed, frozen tonsillar mucosa human tissue sections from healthy and hyper-IgM syndrome (HIGM) donors, respectively. IF/ICC: Fig 4C, mucosal B cells from the colon lamina propria of the HIGM donor. 2. Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth. Cho WG, RJC Albuquerque, ME Kleinman, V Tarallo, A Greco, M Nozaki, MG Green, JZ Baffi, BK Ambati, M De Falco, JS Alexander, A Brunetti, S De Falco, J Ambati. PNAS 106:7137-7142 (2009). IMGENEX products cited.1. IMG-516 (TLR3 pAb): IHC (frozen), Figs 3A,B and S1A, B (mouse corneas and skeletal muscle ) .2. IMG-5348A [TLR3/CD283 (Phospho Tyr 759)]: WB, Fig 4D (mouse primary lymphatic endothelial cell cultures). Note: exposure to 21nt-siRNA-luciferase triggered TLR3 phosphorylation with 5 min after stimulation. .
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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IMG-5348A [Polyclonal Antibody to TLR3/CD283 (Phospho Tyr759)]
