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Product : Antibody >> NF-kB Pathway , Signal Transduction , TNF Family & Death Re.. , Toll-like Receptor Associ.. , Transcription Factors >> TRAF6
      
 

Polyclonal Antibody to TRAF (Tumor Necrosis factor (TNF) associated Factor) 6

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-536
TRAF6Purified
0.1 mg
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Catalog No: IMG-536
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein G Chromatography
Species: Human, Mouse
Host: Rabbit
Application: Western blot analysis: 3-5 ug/ml
Storage: Store at 4°C, stable for 6 months. For long-term storage, store at -20°C.
Recommended
Positive Control:
Human thymus, testis cell lysate 
 

Background
Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). Recently it has been shown that TRANCE/OPGL activates the antiapoptotic serine/threonine kinase Akt/PKB through a signaling complex involving c-Src and TRAF6 (5). Mice deficient in TNF receptor-associated factor 6 (TRAF6) are osteopetrotic with defects in bone remodeling and tooth eruption due to impaired osteoclast function (6). Like TRAF2 and TRAF3, TRAF6 is also essential for perinatal and postnatal survival. These findings establish unexpectedly diverse and critical roles for TRAF6 in perinatal and postnatal survival, bone metabolism, LPS, and cytokine signaling. RANK (TRANCE receptor) interacts with various TRAFs through distinct motifs and activates NF-kB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kB activation (7). Over-expression of TRAF6 activates JNK, p38, or IKK in the absence of extracellular stimulation.

Antigen
Anti-TRAF6 polyclonal antibody was raised against a peptide corresponding to amino acids 436-449 of human TRAF6.

Genebank Info (Protein): NP_665802
Gene ID: 7189
SwissProt: Q9Y4K3
 
TRAF6 Antibody

Western blot analysis of TRAF6 using IMG-536 at 4 ug/ml in A) human thymus cell lysate and B) human testis cell lysate.

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Reference
1. Cheng G, Cleary AM, Ye ZS, Hong DI, Lederman S, and Baltimore D. Science 267: 1494-1498 (1995).
2. Nakane H, Oshima H, Chung W, Williams-Abbit L, Ware CF, Yagita H, and Okumura K. J. Biol. Chem. 271: 14661-14664 (1996).
3. Rothe M, Wong SC, Henzel WJ, and Goeddel DV. Cell 78: 681-692 (1994).
4. Cao Z, Xiong J, Takeuchi M, and Kurama, T. Nature 383: 443-446 (1996).
5. Wong BR, Besser D, Kim N, Arron JR, Vologodskaia M, Hanafusa H, Choi Y. Mol. Cell 6:1041-1049 (1999).
6. Lomaga MA, Yeh WC, Sarosi I, Duncan GS, et al. Genes Dev 13(8): 1015-1024 (1999).
7. Darnay BG, Ni J, Moore PA, Aggarwal BB. J Biol Chem 274 (12): 7724-7731 (1999).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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