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Datasheet
Peptide-affinity Purified Polyclonal Antibody to b-actin (NT) - Loading Control
Cat.No IMG-5401
Description b-actin (NT) - Loading Control
Format Purified, Peptide Affinity
Unit 0.1 mg
Price(USD) 275.00
MSDS
Catalog No
:
IMG-5401
Contents
:
0.1 mg of purified antibody in PBS containing 0.02% sodium azide.
Isotype
:
Rabbit Ig
Clone
:
N/A
Purification
:
Antigen Affinity Chromatography
Species
:
Human, Mouse, Rat
Host
:
Rabbit
Application
Western blot analysis: 1-2 ug/ml Storage Store at 4°C. Recommended Positive Control : HeLa cell lysate can be used as positive control.
Background Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, b-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Antigen
Human b-actin/Beta actin (NT) peptide.
Western blot analysis of b-actin in HeLa cell lysate using IMG-5401 at (A) 0.5, (B) 1 and (C) 2 µg/ml.
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Reference 1. Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.
2. Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing? Nat. Rev. 2005; 6:583-9.
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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