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Peptide-affinity Purified Polyclonal Antibody to AES (NT) |
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| Catalog No: |
IMG-5409-1 |
| Contents: |
0.05 mg of purified antibody in PBS containing 0.02% sodium azide. |
| Isotype: |
Rabbit Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human, Mouse |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 2-4 ug/ml |
| Storage: |
Store at 4°C. |
Recommended
Positive Control: |
293 cell lysate can be used as positive control. |
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Background
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the ?5?1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the ?5?1 integrin. |
Antigen
Human AES / Amino-terminal enhancer of split / GRG / ESP1 / TLE5 (NT) peptide. |
Genebank Info (Protein)
NP_945321 |
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Western blot analysis of AES in 293 cell lysate with AES antibody using IMG-5409 at (A) 2 and (B) 4 µg/ml. |
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Reference
1. Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.
2.
Miyasaka H, Choudhury BK, Hou WE, et al. Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur. J. Biochem. 1993; 216:343-52.
3.
Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16.
4.
Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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