PATHWAY PROFILING ANTIBODIES & REAGENTS

     
Products
Product : Antibody >> DNA Methylation & Rep.. >> AID/AICDA
      
 

Peptide-affinity Purified Polyclonal Antibody to AID/AICDA

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-5410-2
AID/AICDAPurified, Peptide Affinity
0.1 mg
 --
 
Catalog No: IMG-5410-2
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg of purified antibody in PBS containing 0.02% sodium azide.
Isotype: Rabbit Ig
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Human, Mouse
Host: Rabbit
Application: IF/ICC: 10 ug/ml
Western blot analysis: 1-2 ug/ml
Storage: Store at 4C.
Recommended
Positive Control:
Whole cell lysate from Ramos cells can be used as positive control.
 

Background
Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.

Antigen
A synthetic peptide corresponding to 14 amino acids near the carboxy-terminus of human AID was used as immunogen.

Genebank Info (Protein): NP_065712
Gene ID: 57379
 
AID/AICDA Antibody

Western blot analysis of AID in Ramos whole cell lysate with anti-AID using IMG-5410 at 2 g/ml in either the (A) absence or (B) presence of blocking peptide.

AID/AICDA Antibody IMG-5410-2

Immunocytochemistry analysis of AID in Ramos cells using IMG-5410 at 10 ug/ml.

-

Reference
1. Muramatsu M, Sankaranand VS, Anant S, et al. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem. 1999; 274:18470-6.
2. Muramatsu M, Kinoshita K, Fagarasan S, et al. Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme. Cell 2000; 102:553-63.
3. Greeve J, Philipsen A, Krause K, et al. Expression of activation-induced cytidine deaminase in human B-cell Hodgkin lymphomas. Blood 2003; 101:3574-80.
4. Cascalho M. Advantages and disadvantages of cytidine deamination. J. Immunol. 2004; 172:6513-8.
5. Sheehy AM, Gaddis NC, Choi JD, et al. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 2002; 418:646-50.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


Products

Services

Resources

Purchasing

Company

  • What's New
  • Reporter Cells
  • Screening Services
  • Interactive Pathways