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Peptide-affinity Purified Polyclonal Antibody to ACE2 (NT) |
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| Catalog No: |
IMG-5545-1 |
| Contents: |
0.05 mg of purified antibody in PBS containing 0.02% sodium azide. |
| Isotype: |
Rabbit Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 2 ug/ml
Western blot analysis: 0.5-2 ug/ml |
| Storage: |
Store at 4°C. |
Recommended
Positive Control: |
Human kidney or human testis cell lysate can be used as positive control. |
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Background
Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function. |
Antigen
Human ACE2 (NT) peptide. |
Genebank Info (Protein)
NP_068576 |
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Western blot analysis of ACE2 in human kidney lysate with anti-ACE2 (NT) using IMG-5545 at 1 µg/ml. |
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Immunohistochemical staining of human kidney cells using anti-ACE2 (NT) at 2 µg/ml. |
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Reference
1. Donoghue M, Hsieh F, Baronas E, et al. A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotension I to angiotension 1-9. Circ. Res. 2000;87:1-9.
2.
Tipnis SR, Hooper NM, Hyde R, et al. A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase. J Biol. Chem. 2000;275:33238-43.
3.
Li W, Moore MJ, Vasileva N, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 2003;426:450-4.
4.
Crackower MA, Sarao R, Oudit GY, et al. Angiotensin-converting enzyme 2 is an essential regulator of heart function. Nature 2002;417:822-8.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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