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Peptide-affinity Purified Polyclonal Antibody to Acinus (CT) |
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| Catalog No: |
IMG-5547-2 |
| Contents: |
0.1 mg of purified antibody in PBS containing 0.02% sodium azide. |
| Isotype: |
Rabbit Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Host: |
Rabbit |
| Application: |
IF/ICC: 0.5 ug/ml
Western blot analysis: 0.25-0.5 ug/ml |
| Storage: |
Store at 4°C. |
Recommended
Positive Control: |
K562 whole cell lysate. |
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Background
Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS’. |
Antigen
Human Acinus (CT) peptide. |
Genebank Info (Protein)
NP_055792 |
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Western blot analysis of Acinus in K562 whole cell lysate with Acinus antibody using IMG-5547 at 0.5 µg/ml. |
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Immunocytochemical analysis of Acinus in formalin-fixed K562 cells using IMG-5547 at 0.5 µg/ml. |
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Reference
1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.
2. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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