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Polyclonal Antibody to ATR (IN) |
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| Catalog No: |
IMG-5638-1 |
| Contents: |
0.05 mg of purified antibody in PBS containing 0.02% sodium azide. |
| Isotype: |
Rabbit Ig |
| Clone: |
N/A |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 2 ug/ml
Western blot analysis: 0.5-1 ug/ml |
| Storage: |
Store at 4°C. |
Recommended
Positive Control: |
HepG2 cell lysates. |
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Background
The Anthrax toxin receptor (ATR) was initially discovered as the tumor endothelial marker 8 (TEM8). This protein, which exists in three isoforms (36, 40, and 60 kDa), is highly expressed in tumor vessels as well as in the vasculature of developing embryos, suggesting that it may normally play a role in angiogenesis. However, it also acts as the receptor for anthrax toxin. Following the binding of this protein by the protective antigen (PA) of anthrax, PA is cleaved and heptamerizes to form the binding site for both edema factor (EF) and lethal factor (LF). This complex is then endocytosed by the cell; acidification in endosomes allows the release of EF and LF into the cytoplasm where they interfere with MAPK signaling and induce apoptosis. |
Antigen
Human ATR (IN) (TEM8) peptide. |
Genebank Info (Protein)
NP_001175 |
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Western blot analysis of ATR in HepG2 cell lysates with anti-ATR (IN) using IMG-5638 at (A) 0.5, (B) 1, and (C) 2 µg/ml. |
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Immunohistochemical staining of human brain tissue using anti-ATR at 2 µg/ml. |
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Reference
1. Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Can. Res. 2001; 61:6649-6655.
2. Bradley KA, Mogridge J, Mourez M, et al. Identification of the cellular receptor for anthrax toxin. Nature 2001; 414:225-9.
3.
Molloy S, Bresnahan PA, Thomas G, et al. Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen. J. Biol. Chem. 1992; 267:16396-402.
4.
Duesbery N, Webb C, Vande Woude G, et al. Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor. Science 1998; 280:734-6.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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