PATHWAY PROFILING ANTIBODIES & REAGENTS

     
Products
Product : Antibody >> Apoptosis , Cancer Antibodies , Neurobiology , Nuclear & Cytoplasmic.. >> DAXX
      
 

Polyclonal Antibody to DAXX

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-5723
DAXXSera
0.05 mL
 --
 
Catalog No: IMG-5723
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 50 ul neat serum containing 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Clone: N/A
Purification: Neat Serum
Species: Cow, Dog, Human, New World monkey, Rat
Predicted React: Mouse
Host: Rabbit
Application: IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)
IHC (paraffin): 1:1000-1:5000
IP: 1:50-1:200
Western blot analysis: 1:1000-1:2000
Storage: Aliquot and store at -20°C. Avoid repeated freeze-thaw cycles.
Recommended
Positive Control:
prostate cancer, thymus, testes
 

Background
Daxx (death-domain-associated protein) was originally identified as a cytoplasmic protein that binds to the death domain of the transmembrane death receptor Fas.  It is now that known that the a large porportion of Daxx molecules are nuclear and associate with the promyelocytic leukaemia nuclear body (PML-NB) and other subnuclear domains (reviewed in Salomoni and Khelifi).  The promyelocytic leukemia protein Pml is an essential component of the PML-NB.  Data suggests that certain stimuli including Fas stimulation, causes Daxx to be translocated from the nucleus to the cytoplasm.    Daxx is ubiquitously expressed, and particularly high levels of Daxx have been reported in the thymus and testes.  At the cellular level Daxx may be found in cytoplasm and within heterochromatic regions of the nucleus.  Daxx has been reported to interact and co-localize with Pml in the nucleus of tumor cell lines and primary cells.  Pml is necessary for Fas-induced cell death and Daxx pro-apoptotic functions. Daxx is thought to have both pro- and anti-apoptotic functions depending on the stimulus and the cell type. Daxx pro-apoptotic functions appear to occur in both the cytoplasm and nucleus. Although the mechanisms remain to be fully elucidated,  research indicates that Daxx plays a role in the pathology of human diseases including cancer and neurodegerative disorders.  Human Daxx is a 740 amino acid protein (GenBank no. gi/62898369).

Antigen
A synthetic peptide corresponding to amino acids 272-291 (ERLINKPGPDTFPDYGDVLR, GenBank no. gi/62898369) of human DAXX was used as immuogen.  This sequence is 100% consevered in dog, cow, rat, monkey and 95% conserved in mouse.

 
DAXX Antibody IMG-5723

Formalin-fixed paraffin-embedded tissue sections of human prostate cancer (A and B) stained for Daxx expression using IMG-5723 at 1:2000.  Hematoxylin-eosin counterstain.

-

Reference
1. Salomoni P and AF Khelifi. 2006. Daxx:death or survival protein? TRENDS in Cell Biol. 16:97-104.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


Products

Services

Resources

Purchasing

Company

  • What's New
  • Reporter Cells
  • Screening Services
  • Interactive Pathways