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Datasheet
Peptide-affinity Purified Polyclonal Antibody to b-Catenin (NT)
Cat.No IMG-5778
Description b-Catenin (NT)
Format Purified, Peptide Affinity
Unit 0.1 mL
Price(USD) 295.00
MSDS
Catalog No
:
IMG-5778
Contents
:
0.1 ml of purified antibody in PBS, 50% gylcerol, 1 mg/ml BSA, and 0.05% sodium azide.
Isotype
:
Rabbit Ig
Clone
:
N/A
Purification
:
Antigen Affinity Chromatography
Species
:
Human, Mouse, Rat
Host
:
Rabbit
Application
IP: 5-10ul Western blot analysis: 1:1000 Storage Store at -20°C. Recommended Positive Control : Hct116 src transformed cells treated with pervanadate. Human endothelial, A431, and HeLa cells may also be used.
Background b-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of b-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between a- and b-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of b-Catenin. Src family kinases can also phosphorylate tyrosine 654 in the C-terminal armadillo repeat of b-Catenin. This phosphorylation regulates b-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of b-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion.
Antigen
b-Catenin synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human b-Catenin.
Application Notes
This human sequence is highly conserved in rat and mouse b-Catenins. This antibody detects a 92kDa protein corresponding to the molecular mass of b-Catenin on SDS-PAGE immunoblots of Hct116 src transformed cells. Similar results were seen in human endothelial, A431, and HeLa cells.
Western blot analysis of Hct116 src transformed cells (20 ug/lane) serum starved overnight or pervanadate treated (1 mM, 30 minutes) using IMG-5778 (lane 1) or anti-b-Catenin (Tyr142), IMG-5779 (lane 2).
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Related Products
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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IMG-5778 [Peptide-affinity Purified Polyclonal Antibody to b-Catenin (NT)]
