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Polyclonal Antibody to TLR4 (Toll-like receptor 4)/CD284 |
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| Catalog No: |
IMG-578A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse, Rhesus Monkey |
| Host: |
Rabbit |
| Application: |
IF/ICC: 2-5 ug/ml. See Bernardino et al (2008) for details.
IHC (p): 5 ug/ml
Western blot analysis: 2-5 ug/ml |
| Storage: |
Store at 4oC, stable for 6 months. For long-term storage, store at -20oC. |
Recommended
Positive Control: |
Recombinant protein, RAW, Daudi |
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Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90 kDa (6). |
Antigen
This antibody was developed against a sythetic peptide corresponding to amino acids 39-56 (CMDQKLSKVPDDIPSSTK) of mouse TLR4, GenBank noCAD83014.1. |
Application Notes
1. This antibody was tested against partial recombinant mouse TLR4 (extracellular portion), and a 75-80 kDa band was observed. Full-length TLR4 is observed at approximately 90 kD.
2. Zager et al (2006, Fig. 10) observed TLR4 as an ~95 kDa doublet which is thought to represent different degrees of glycosylation. See Product Citation (Renal Physiol 291:F546-F556) for additional information.
3. Presumptive TLR4 30 and 60 kDa cleavage fragments are described in Zagar et al 2006. See Product Citation (Am J Physiol Renal Physiol F304-F312) for additional information. |
Genebank Info (Protein)
NP_612564 |
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Western blot analysis of TLR4 using IMG-578A on A) 0.1 ug/lane partial recombinant mouse TLR4 protein (tested at 2 ug/ml) and B) RAW cell lysate and C) Daudi cell lysate (tested at 5 ug/ml on tumor cell lysate). |
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Immunohistochemical analysis of TLR4 in mouse kidney tissue using TLR4 antibody at 5 ug/ml. |
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Reference
1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998).
2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001).
4. Takeuchi O., Hoshino K, Kawai T, Sanjo H, Takada H, Ogawa K, Takeda K and Akira S. Immunity 11: 443 (1999).
5. Poltorak A, Riccardi-Castagnoli P, Citterio S, and Butler B. Proc. Natl. Acad. Sci USA 97: 2163-2167 (2000).
6. Medzhitov R, Preston-Hurlburt P and Janeway CA Jr. Nature 388 (6640), 394-397 (1997).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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