Polyclonal Antibody to beta Tubulin

Background
Alpha and beta Tubulin are the most common members of the tubulin family of proteins. Dimers of alpha and beta Tubulin assemble to form microtubules. An antibody against beta tubulin is often used as a protein loading control in western blotting.

Antigen
A synthetic peptide corresponding to amino acids 417-435 (DLVSEYQQYQDATADEQGE) of human beta Tubulin was used as immunogen for this antibody, GenBank no. gi|27227551|gb|AAN85571.1|. This sequence is 100% conserved between multiple species including bovine, chicken, dog, monkey, mouse, rat, and xenopus.

Application Notes
The amino acid sequence used as immunogen for this antibody is 100% homologous in beta I and beta II Tubulin.

Reference
1. Yeh,T.S., Hsieh,R.H., Shen,S.C., Wang,S.H., Tseng,M.J., Shih,C.M. and Lin,J.J.  Nuclear betaII-tubulin associates with the activated notch receptor to modulate notch signaling.  Cancer Res. 64 (22), 8334-8340 (2004).
2. Takahashi,M., Shiraishi,H., Ishibashi,Y., Blade,K.L., McDermott,P.J., Menick,D.R., Kuppuswamy,D. and Cooper,G. IV.  Phenotypic consequences of beta1-tubulin expression and MAP4 decoration of microtubules in adult cardiocytes.  Am. J. Physiol. Heart Circ. Physiol. 285 (5), H2072-H2083 (2003).
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6. Yaroslav Blume, Alla Yemets, Yarina Sheremet, Alexey Nyporko, Vadym Sulimenko, Tetyana Sulimenko, Pavel Dráber. Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells. BMC Plant Biol. 2010; 10: 29.
7. Hyo Jung Nam, Jin Ku Kang, Sung-Kuk Kim, Keun Jae Ahn, Heon Seok, Sang Joon Park, Jong Soo Chang, Charalabos Pothoulakis, John Thomas Lamont, Ho Kim. Clostridium difficile Toxin A Decreases Acetylation of Tubulin, Leading to Microtubule Depolymerization through Activation of Histone Deacetylase 6, and This Mediates Acute Inflammation. J Biol Chem. 2010 October 22; 285(43): 32888–32896.