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Polyclonal Antibody to Chaperonin/Hsp60 |
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| Catalog No: |
IMG-5822A |
| Contents: |
100 ug in 200 ul PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Protein G Chromatography |
| Species: |
Bovine, Broad, Chicken, Chimpanzee, Dog, Human, New World monkey |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 0.2-1 ug/ml
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| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
HeLa, Jurkat cell lysate |
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Background
Principal Names: 60KDa Chaperonin (CPN60); 60KDa Heat shock protein (HSP60); GROEL; Mitochondrial matrix protein P1; P60 lymphocyte protein; SPG13; HuCHA60; Official Gene Symbol: HSPD1 Gen Bank Accession Number: NP_002147 Gene ID: 3329 (Human) Gene Map Locus: 2q33.1 (Human)
HSP60, a 60KDa member of Chaperonin family, is a mitochondrial protein that participates in protein folding, transport and secretion. HSP60 is a multi-functional protein whose roles include accelerating the maturation of pro-caspase 3 during apoptosis, LPS-binding, acting as a ligand for innate immune system and also binding to the surface of innate immune cells and stimulating proinflammatory reactivity. It induces the expression of IL-12 and IL-15 and also induces release of TNF-alpha, NO and IL-6. Reports suggest interaction of Hsp60 with integrin molecules during metastasis. Mutations in Hsp60 gene cause autosomal recessive spastic paraplegia 13. Hsp60 has been found to be involved in development of auto-immune disorders such as Rheumatoid arthritis, insulin-dependent diabetes mellitus, systemic sclerosis and atherosclerosis. |
Antigen
A portion of amino acids 350-400 of human Hsp60 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in a broad range of species including human, dog, mouse, rat, bovine, chicken, chimp, monkey, and orangutan. |
| Genebank Info (Protein): NP_955472 |
| Gene ID: 3329 |
| SwissProt: P10809 |
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Western blot analysis of Hsp60 in the 1) absence and 2) presence of imminizing peptide in HeLa cell lysate and the 3) absence and 4) presence of immunizing peptide in Jurkat cell lysate using IMG-5822A at 0.2 ug/ml. |
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Reference
1. Satoh,J., Onoue,H., Arima,K. and Yamamura,T. The 14-3-3 protein forms a molecular complex with heat shock protein Hsp60 and cellular prion protein. J. Neuropathol. Exp. Neurol. 64 (10), 858-868 (2005)
2. Jamin,C., Dugue,C., Alard,J.E., Jousse,S., Saraux,A., Guillevin,L., Piette,J.C. and Youinou,P. Induction of endothelial cell apoptosis by the binding of anti-endothelial cell antibodies to Hsp60 in vasculitis-associated systemic autoimmune diseases. Arthritis Rheum. 52 (12), 4028-4038 (2005)
3. Cohen-Sfady,M., Nussbaum,G., Pevsner-Fischer,M., Mor,F., Carmi,P., Zanin-Zhorov,A., Lider,O. and Cohen,I.R. Heat shock protein 60 activates B cells via the TLR4-MyD88 pathway. J. Immunol. 175 (6), 3594-3602 (2005)
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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