Polyclonal Antibody to Tar (HIV-1) RNA binding protein 2 (TRBP2) 

Background
Principal Names: TAR (HIV) RNA-binding protein 2; HIV-Transactivating response RNA-binding protein; TRBP; Official Gene Symbol: TARBP2   TRBP belongs to the family of dsRNA-binding proteins. It consists of highly conserved 2 dsRNA-binding domains and C-terminal basic region. It is a unique cellular protein capable of binding to HIV-TAR RNA and stimulates expression of HIV-1 long terminal repeats. It also inhibits the host antiviral and anti-proliferative mechanisms by directly binding to IFN-regulated dsRNA-dependent protein kinase (PKR). TRBP associates with Ago2 protein and forms an integral component of Dicer-containing complex. It has a role in micro RNA processing and in RISC assembly. Other functions of TRBP include promoting cellular growth and tumorigenesis.

Antigen
A portion of amino acids 150-200 of human TRBP2 was used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in human (isoforms a and b), mouse, rat, bovine, chimp, and zebrafish.  Human isoform a is a 366 amino acid protein, and human isoform b is a 345 amino acid protein.

Genebank Info (Protein)
NP_599151

Reference
1. Battisti,P.L., Daher,A., Bannwarth,S., Voortman,J., Peden,K.W., Hiscott,J., Mouland,A.J., Benarous,R. and Gatignol,A. Additive activity between the trans-activation response RNA-binding protein, TRBP2, and cyclin T1 on HIV type 1 expression and viral production in murine cells AIDS Res. Hum. Retroviruses 19 (9), 767-778 (2003)
2. Daher,A., Longuet,M., Dorin,D., Bois,F., Segeral,E., Bannwarth,S., Battisti,P.L., Purcell,D.F., Benarous,R., Vaquero,C., Meurs,E.F. and Gatignol,A.  Two dimerization domains in the trans-activation response RNA-binding protein (TRBP) individually reverse the protein kinase R inhibition of HIV-1 long terminal repeat expression J. Biol. Chem. 276 (36), 33899-33905 (2001)