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Polyclonal Antibody to p53 |
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| Catalog No: |
IMG-583 |
| Contents: |
Antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Rabbit Serum |
| Species: |
Human, Insects, Mouse, Rat |
| Predicted React: |
Cat, Dog, Gorilla, Most Mammals, New World monkey, Pig, Rabbit, White Whale |
| Host: |
Rabbit |
| Application: |
ChIP: dilutions determined by researcher
Gel Shift: dilutions determined by researcher
IP:
Western blot analysis: 1:500-1:2000 |
| Storage: |
Store at 4°C for up to six months. For long-term storage, store at -20°C. |
Recommended
Positive Control: |
293 |
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Background
The nuclear phosphoprotein p53 acts as a tumor suppressor protein, arresting cell cycle progression or inducing apoptosis in response to DNA damage or other cell stresses. |
Antigen
A synthetic peptide corresponding to amino acids 367-381 (SHLKSKKGQSTSRHK) of human p53 was used as immunogen; GenBank Accession No: NP_000537.2. This peptide sequence is highly conserved (90-95%) among many mammalian species. |
Genebank Info (Protein)
NP_001119584 |
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Western blot analysis for human p53 using IMG-583 at 1:1000 dilution on 15 µg of 293 cell lysate. |
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Reference
1. Lambkin, H.A. et al. 1994. J. Pathol. 172: 13-18.
2. Bonsing, B.A. et al. 1997. Cytometry 28: 11-24.
3. Vogelstein, B. 1990. Nature. 348: 681-682.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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