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Polyclonal Antibody to NFkB p50 |
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| Catalog No: |
IMG-5845A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Protein G Chromatography was used to purify this NFkB antibody |
| Species: |
Chimpanzee, Human, Rhesus Monkey |
| Host: |
Rabbit |
| Application: |
Western blot analysis: use antibody at 0.5-2 ug/ml |
| Storage: |
Store NFkB p50 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C. |
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Background
The active nuclear form of the NFkB transcription factor complex is composed of two DNA binding subunits, NFkB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of NFkB p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65. |
Antigen
A portion of amino acids 1-50 of human NFkB p50 was used as the immunogen for this antibody. |
Application Notes
The amino acid sequence used as immunogen for this NFkB p50 antibody is 100% homologous in human (multiple isoforms), chimp (multiple isoforms) and rhesus monkey. Based on NCBI BLAST, it will not cross with mouse or rat. |
| Genebank Info (Protein): NP_078820 |
| Gene ID: 79594 |
| SwissProt: Q969V5 |
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Western blot analysis of NFkB p50 in Raji lysate in the 1) absence and 2) presence of immunizing peptide using NFkB p50 antibody at 0.5 ug/ml. |
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Reference
1. Xing L, et al. J Bone Miner Res 17(7):1200-1210 (2002)
2. Kim BY, et al. Oncogene. 2002 Jul 4;21(29):4490-4497 (2002).
3. Tanaka H, et al. Mol Cell 9(5):1017-1029 (2002).
4. Jiang HY, et al. J Virol. 76(11):5737-5747 (2002).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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