Product : Antibody >> Cancer Antibodies , Chromatin , Kinases & Phosphatase.. , New Antibodies , Tumor BioMarkers >> Brd4/HUNK1

Polyclonal Antibody to Brd4 (HUNK1)

Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
Brd4/HUNK1 Purified
0.1 mg
Catalog No: IMG-5859A
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Contents: 100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein G Chromatography
Species: Chimpanzee, Human, Mouse, Rat
Host: Rabbit
Application: Western blot analysis: use antibody at 2-5 ug/ml
Storage: Store Brd4 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C.

Principal Names: Bromodomain-containing protein 4 (Brd4); HUNK1 protein; MCAP; Chromosome-associated protein (CAP) Official Gene Symbol: Brd4  Gene ID: 23476 (Human) Gene Map Locus: 19p13.1 (human)  Brd4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; longer variant has a C-terminus region with a praline and glutamine-rich domains. Brd4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. Brd4 acts at different stages of cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. Brd4 functions as a cellular target for E2 protein of papillomavirus.

A portion of amino acids 150-200 of human Brd4 was used as the immunogen for this antibody.

Application Notes
The amino acid sequence used as immunogen for this antibody is 100% homologous in human, chimp, mouse and rat.

Brd4 is expressed in two forms, the long form is approximately 1400 amino acids and the short form is approximately 722 amino acids.

Genebank Info (Protein): NP_490597
Gene ID: 23476
Brd4/HUNK1 Antibody

Western blot analysis of Brd4 in human HeLa lysate in the 1) absence and 2) presence of immunizing peptide and C) mouse NIH 3T3 lysate using Brd4 antibody at 3 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.


1. Maruyama Mol Cell Biol 22 (18) 6509-6520 (September 2002).
2. Farina Mol Cell Biol 24 (20) 9059-9069 (October 2004).
3. McPhillips J Virol 80 (19) 9530-9543 (October 2006).

Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.






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