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Polyclonal Antibody to Farnesyltransferase/CAAX box |
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| Catalog No: |
IMG-5892A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein G Chromatography was used to purify the FNTA antibody |
| Species: |
Chicken, Chimpanzee, Dog, Human, Rat |
| Predicted React: |
Mouse |
| Host: |
Rabbit |
| Application: |
IHC(paraffin): 10 ug/ml
Western blot analysis: 1-3 ug/ml |
| Storage: |
Store FNTA antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
Mouse colon cell lysate |
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Background
Principal Names: Farnesyltransferase, CAAX box (FNTA); alphaCAAX farnesyltransferase alpha subunit, FPTA, FTase-alpha, GGTase-I-alpha, Protein farnesyl transferase/ geranyl geranyl transferase type I alpha subunit(PGGT1A), Ras proteins prenyl transferase alpha, Type I protein geranyl-geranyl transferase alpha subunit; Official Gene Symbol: FNTA Gen Bank Accession Number: NP_002018, Gene ID: 2339 (human) Gene Map Locus: 8p11 (human) Prenylation is a common intracellular posttranslational protein modification catalyzed by prenyltransferases. The best characterized among these prenyltransferases is the CAAX farnesyl transferase (2.5.1.21), which is alpha-beta heterodimeric enzyme. It catalyzes the attachment of a farnesyl group from farnesyl phosphate to cysteine residues at the fourth position from the C terminus of proteins that end in the CAAX box. FNTA or the alpha subunit of farnesyl transferase is shared by the geranyl-geranyl protein transferases. Apart from transfer of farnesyl and geranyl groups to proteins, FNTA has been proposed to regulate intracellular signaling pathways involving p21ras. Reports have shown that FNTA interacts with TGF-beta and activin-type I receptors. |
Antigen
A portion of amino acids 150-250 of human FNTA was used as the immunogen for the antibody. |
Application Notes
The amino acid sequence used as immunogen for the FNTA antibody is 100% homologous in human (isoforms CRA_a, _b, _c), rat, dog, chicken, and chimp (isoforms 1, 2, 3), and 93% homologous in mouse. |
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Western blot analysis of FNTA in mouse colon cell lysate in the 1) absence and 2) presence of immunizing peptide using FNTA antibody at 2 ug/ml. |
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IHC analysis of human prostate using FNTA antibody at 10 ug/ml. |
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Reference
1. Wang et.al Science 271; 1120-1122 (1996)
2. Lane et.al J Lipid Res 47; 681-699 (2006)
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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