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Polyclonal Antibody to Importin-8 (RAN-binding protein 8) |
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| Catalog No: |
IMG-5894A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Protein G chromatography was used to purify the Importin-8 antibody. |
| Species: |
Chimpanzee, Human, New World monkey |
| Predicted React: |
Mouse, Dog, Cow, Chicken |
| Host: |
Rabbit |
| Application: |
IF/ICC: 5-10 ug/ml
Western blot analysis: 0.5-2 ug/ml |
| Storage: |
Store Importin-8 antibody at 4°C, stable for 6 months. For long term storage, aliquot and store antibody at -20°C. |
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Background
Importin-8 is a 1037 amino acid nuclear protein with a RAN binding site. It belongs to RAN-binding protein super family and hence shares an N-terminal sequence motif with importin-beta that appears to account for RAN GTP binding. Importin-8 binds to the nuclear pore complex (NPC) and is well known as a transport carrier that mediates nuclear import of ribosomal proteins with a classical nuclear localization signal. Importin-8 works by binding to the nuclear pore complex and, along with RANGTP and RANBP1, inhibits the GAP stimulation of the RAN GTPase. Along with transportin, Importin-8 also helps in the nuclear import of SRP19. |
Antigen
A portion of amino acids 850-900 of human Importin-8 was used as immunogen for the antibody. |
Application Notes
The amino acid sequence used as immunogen for the Importin-8 antibody is 100% homologous in human and chimpanzee and monkey, 97% in cow and dog, 92% in mouse, and 86% homologous in chicken and opossum. |
Genebank Info (Protein)
NP_006381 |
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Western blot analysis of Importin-8 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide using Importin-8 antibody at 1 ug/ml. |
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ICC staining of HeLa cells using Importin-8 antibody at 5 ug/ml. |
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Reference
1. Dean, KA. et al. J. Cell. Sci. 114:3479-3485 (2001).
2. Gorlich, D. et al. J. Cell Biol. 138:65-80 (1997).
3. Johansson, S. et al. Brain. Res. 1132:20-28 (2007).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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