|
|
|
| |
Monoclonal Antibody to human CD4 (Clone RPA-T4) |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-5916A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
RPA-T4 |
| Purification: |
Protein G Chromatography |
| Species: |
Human |
| Host: |
Mouse |
| Application: |
FA (Neutralization): Reported in literature
Flow (Cell Surface): 0.1-0.25 ug per 1x10^6 cells
IHC: Reported in literature |
| Storage: |
Store CD4 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
|
 |
|
|
|
|
|
|
Background
CD4 is an Immunoglobulin Superfamily protein and an accessory protein for MHC class-II antigen/ T-cell receptor interaction. It may regulate T-cell activation. It plays a more general role in mediating cell recognition events than merely those of cellular immune response. The T4 molecule serves as a receptor for the human immunodeficiency virus. A Type I membrane protein, it is expressed on T lymphocytes, B-cells, macrophages, and granulocytes. It is also expressed in a developmentally regulated manner in specific regions of the brain. |
Antigen
PHA-stimulated human PBMC. |
Application Notes
RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation. The RPA-T4 clone reacts with CD4, a 59 kDa single-chain transmembrane glycoprotein [receptor for human HIV virus] present on T-helper/inducer cell populations. This antibody binds to the D1 domain of CDR1 and CDR3 epitopes. The CD4 antigen and reacts with approvimately 80% of thymocytes and 45% of peripheral blood lymphocytes. CD4 is also present in low density on peripheral blood monocytes. Clone RPA-T4 is widely published in literature, see Knapp W, Dorken B, Rieber E P, et all, ed. Also see Schlossman SF, Boumsell L, Gilks W, et al, ed. |
Genebank Info (Protein)
NP_000607 |
| |
|
|
Cell surface flow analysis of CD4 in human PBMC using IMG-5916A at 0.25 ug/10^6 cells. Cells were stained with primary antibody followed by a PE-conjugated goat anti-mouse secondary antibody (20103). Green represents isotype control (20109); red represents anti-CD4 antibody. Cells in the lymphocyte gate were used for analysis. |
|
|
|
|
|
| - |
 |
|
Reference
1. Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
