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Product : Antibody >> CD Markers , Innate Immunology , New Antibodies , Regulatory T Cells (Treg) , Th17 Antibodies >> CD4 (L3T4)
      
 

Monoclonal Antibody to human CD4 (Clone RPA-T4)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-5916A
IMG-5916A-25UG
CD4 (L3T4)Purified
0.1 mg
25 ug
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Catalog No: IMG-5916A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: CD4 antibody at 0.5 mg/ml in PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: RPA-T4
Purification: Protein G Chromatography
Species: Human
Host: Mouse
Application: FA (Neutralization): Reported in literature
Flow (Cell Surface): 0.1-0.25 ug per 1x10^6 cells
IHC (frozen): Reported in literature
Storage: Store CD4 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C.
 

Background
CD4 is an Immunoglobulin Superfamily  protein and an accessory protein for MHC class-II antigen/ T-cell receptor interaction. It may regulate T-cell activation. It plays a more general role in mediating cell recognition events than merely those of cellular immune response. The T4 molecule serves as a receptor for the human immunodeficiency virus. A Type I membrane protein, it is expressed on T lymphocytes, B-cells, macrophages, and granulocytes. It is also expressed in a developmentally regulated manner in specific regions of the brain.

Antigen
PHA-stimulated human PBMC.

Application Notes
RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation. The RPA-T4 clone reacts with CD4, a 59 kDa single-chain transmembrane glycoprotein [receptor for human HIV virus] present on T-helper/inducer cell populations. This antibody binds to the D1 domain of CDR1 and CDR3 epitopes. The CD4 antigen and reacts with approvimately 80% of thymocytes and 45% of peripheral blood lymphocytes. CD4 is also present in low density on peripheral blood monocytes. Clone RPA-T4 is widely published in literature, see Knapp W, Dorken B, Rieber E P, et all, ed. Also see Schlossman SF, Boumsell L, Gilks W, et al, ed.

Genebank Info (Protein): NP_000607
Gene ID: 920
SwissProt: P01730
 
CD4 (L3T4) Antibody IMG-5916A

Cell surface flow analysis of CD4 in human PBMC using IMG-5916A at 0.25 ug/10^6 cells. Cells were stained with primary antibody followed by a PE-conjugated goat anti-mouse secondary antibody (20103). Green represents isotype control (20109); red represents anti-CD4 antibody. Cells in the lymphocyte gate were used for analysis.

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Reference
1. Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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