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Product : Antibody >> CD Markers , Innate Immunology , Regulatory T Cells (Treg) , Th17 Antibodies >> CD4 (L3T4)
      
 

Monoclonal Antibody to human CD4 FITC Conjugate (Clone RPA-T4)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-5916C
IMG-5916C-25T
CD4 (L3T4)FITC
100 Tests
25 Tests
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Catalog No: IMG-5916C
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: CD4 antibody at 10 ul/test in 10 mM Tris, 150 mM NaCl, containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: RPA-T4
Purification: Protein G Chromatography
Species: Human
Host: Mouse
Application: Flow (Cell Surface): 10 ul/106 cells (0.1 ug)
Storage: Store at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE.
 

Background
CD4 is an Immunoglobulin Superfamily  protein and an accessory protein for MHC class-II antigen/ T-cell receptor interaction. It may regulate T-cell activation. It plays a more general role in mediating cell recognition events than merely those of cellular immune response. The T4 molecule serves as a receptor for the human immunodeficiency virus. A Type I membrane protein, it is expressed on T lymphocytes, B-cells, macrophages, and granulocytes. It is also expressed in a developmentally regulated manner in specific regions of the brain.

Antigen
PHA-stimulated human PBMC.  

Application Notes

RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation. The RPA-T4 clone reacts with CD4, a 59 kDa single-chain transmembrane glycoprotein [receptor for human HIV virus] present on T-helper/inducer cell populations. This antibody binds to the D1 domain of  CDR1 and CDR3 epitopes. The CD4 antigen and reacts with approvimately 80% of thymocytes and 45% of peripheral blood lymphocytes. CD4 is also present in low density on peripheral blood monocytes.Clone RPA-T4 is widely published in literature, see Knapp W, Dorken B, Rieber E P, et all, ed. Also see Schlossman SF, Boumsell L, Gilks W, et al, ed.

Genebank Info (Protein): NP_000607
Gene ID: 920
 
CD4 (L3T4) Antibody IMG-5916C

Cell surface flow cytometric analysis of CD4 in 1x106 human PBMC using 10 ul (0.1 ug) of IMG-5916C. Green represents isotype control (IMGENEX, 20105); red represents anti-CD4 antibody. Imgenex's cell surface staining flow assay kit (IMGENEX, 10084K) was used to test this product. Propidium iodide negative lymphocyte population gated for analysis.

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Reference
1. Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.


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