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Monoclonal Antibody to human CD4 Azide Free (Clone RPA-T4) |
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| Catalog No: |
IMG-5916E |
| Contents: |
100 ug in 200 ul PBS. Filter sterilized. Sodium azide free. Endotoxin level < 0.06 EU/ug. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
RPA-T4 |
| Purification: |
Protein G Chromatography |
| Species: |
Human |
| Host: |
Mouse |
| Application: |
FA (Neutralization): Reported in literature
Flow (Cell Surface): 0.1-0.2 ug/10^6 cells
IHC: Reported in literature |
| Storage: |
Aliquot and store frozen at -20°C. |
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Background
CD4 is an Immunoglobulin Superfamily protein and an accessory protein for MHC class-II antigen/ T-cell receptor interaction. It may regulate T-cell activation. It plays a more general role in mediating cell recognition events than merely those of cellular immune response. The T4 molecule serves as a receptor for the human immunodeficiency virus. A Type I membrane protein, it is expressed on T lymphocytes, B-cells, macrophages, and granulocytes. It is also expressed in a developmentally regulated manner in specific regions of the brain. |
Antigen
PHA-stimulated human PBMC. |
Application Notes
RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation. The RPA-T4 clone reacts with CD4, a 59 kDa single-chain transmembrane glycoprotein [receptor for human HIV virus] present on T-helper/inducer cell populations. This antibody binds to the D1 domain of CDR1 and CDR3 epitopes. The CD4 antigen and reacts with approvimately 80% of thymocytes and 45% of peripheral blood lymphocytes. CD4 is also present in low density on peripheral blood monocytes.Clone RPA-T4 is widely published in literature, see Knapp W, Dorken B, Rieber E P, et all, ed. Also see Schlossman SF, Boumsell L, Gilks W, et al, ed. |
Genebank Info (Protein)
NP_000607 |
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Flow cytometric analysis of CD4 in human PBMCs using 0.1 ug of IMG-5916E (clone RPA-T4) per approximately 1x10^6 cells. Secondary antibody is goat anti-mouse PE. |
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Reference
1. Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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