Monoclonal Antibody to CD25 (Clone PC61)

Background
CD25 is a 55KDa transmembrane glycoprotein consisting of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. It is expressed during early stages of thymocytes development and in activated CD4+ memory T-lymphocytes. CD25 heterodimerizes with IL2R-beta and IL-2R gamma and forms the tripartite high affinity IL-2 receptor complex. It increases the specificity and affinity of IL-2 receptor complex for IL-2 ligand and is necessary for receptor clustering required for augmentation of IL-2 signaling. Mutations in CD25 result in severe immunodeficiency.

Antigen
The B6.1 mouse cytotoxic T cell line was used as immunogen for this antibody.

Application Notes
Mouse splenocytes were stimulated with 5 ug/ml ConA for three days prior to testing.

Reference
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3. Erber et al. Am J Clin Pathol 89: 645, 1988.
4. Sharfe et.al PNAS 94; 3168-3171 (April 1997)
5. Vamosi et.al PNAS 101 (30); 11082-11087 (July 27, 2004)
6. Z Wang, B-Y Shi, Y-Y Qian, M Cai, Q Wang. Short-term anti-CD25 monoclonal antibody administration down-regulated CD25 expression without eliminating the neogenetic functional regulatory T cells in kidney transplantation. Clin Exp Immunol. 2009 March; 155(3): 496–503.
7. Cintia S De Paiva, Kyung-Chul Yoon, Solherny B Pangelinan, Sapa Pham, Larry M Puthenparambil, Eliseu Y Chuang, William J Farley, Michael E Stern, De-Quan Li, Stephen C Pflugfelder. Cleavage of functional IL-2 receptor alpha chain (CD25) from murine corneal and conjunctival epithelia by MMP-9. J Inflamm (Lond) 2009; 6: 31.