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Peptide-affinity Purified Polyclonal Antibody to IL-33 (IL-1F11) |
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| Catalog No: |
IMG-6016A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic |
| Isotype: |
Rabbit IgG |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Chimpanzee, Human |
| Predicted React: |
Mouse, Dog, Cow |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 0.5-2 ug/ml |
| Storage: |
Store antibody at 4°C, stable for 6 months. For long term storage, aliquot and store antibody at -20°C. |
Recommended
Positive Control: |
HUVEC cell lysate |
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Background
IL-33 is a 270 amino acid highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. It is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. It is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer’s patches and mesenteric lymph nodes and is almost undetectable in placenta. |
Antigen
A portion of amino acids 50-100 of human IL-33 was used as the immunogen for this antibody. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a and CRA_b) and
chimpanzee, 84% in cow, 80% in dog and 72% homologous in mouse. |
Genebank Info (Protein)
NP_254274 |
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Western blot analysis of IL-33 using IL-33 polyclonal antibody. HUVEC cell lysate probed with IL-33 antibody at 1 ug/ml in the 1) absence and 2) presence of immunizing peptide. |
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Reference
1. Carriere, V. et al. Proc. Natl. Acad. Sci. USA. 104:282-287 (2007).
2. Baekkevold, ES. et al. Am. J. Path. 163:69-79 (2003).
3. Schmitz, J. et al. Immunity. 23:479-490 (2005).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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