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Product : Antibody >> New Antibodies , Stem Cell Markers >> DAZL
      
 

Polyclonal Antibody to DAZL

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6027A
DAZLSera
0.1 mL
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Catalog No: IMG-6027A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ul of crude rabbit sera with 50% glycerol and 0.025% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Clone: N/A
Species: Chimpanzee, Cow, Dog, Human, New World monkey, Mouse
Predicted React: Chicken
Host: Rabbit
Application: Western blot analysis: 1:5000 dilution
Storage: Store at 4C, stable for 6 months. For long term storage, aliquot and store at -20C.
Recommended
Positive Control:
Mouse testis lysate
 

Background
Protein expression pattern show that DAZL is involved in mitosis and meiosis of human germ cell. The DAZL gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females .The protein is expressed in germ cells of diverse mammalian species. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In humans, DAZL is expressed in primordial germ cells, adult testis, and the ovary. It plays a central role during spermatogenesis. Defects in DAZL may cause infertility due to severe oligozoospermia (az) and non obstructive azoospermia. The concentrations of DAZL transcripts were lower in men with spermatogenic failure. Drosophila and mouse mutants that have lost DAZ homologous gene function found to be azoospermic, just as with human azoospermia DAZ. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome.

Antigen
A portion of amino acid 50-100 of mouse DAZL was used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in human (isoform CRA_a and CRA_b), chimpanzee, monkey, cow, dog and mouse, 66% homologous in chicken.

Genebank Info (Protein): NP_001342
Gene ID: 1618
SwissProt: Q92904
 
DAZL Antibody

Western blot analysis of DAZL in mouse testis lysate. Lane 1 shows pre-immune sera. Lane 2 shows IMG-6027A tested at 1:5000 on mouse testis lysate.

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Reference
1. Kuo, PL. et.al   Fertil. Steril. 81:1034-1040 (2004).
2. Brekhman, V. et al. Mol. Hum. Reprod. 6:465-468 (2000).
3. Teng, YN. et al. J. Clin. Endocr. Metab. 87:5258-5264 (2002).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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