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Peptide-affinity Purified Polyclonal Antibody to Hyaluronan and proteoglycan link protein 3 (HAPLN3) |
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| Catalog No: |
IMG-6057A |
| Contents: |
100 ug in 200 ul PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Chimpanzee, Human |
| Predicted React: |
New World monkey, Dog, Cow |
| Host: |
Rabbit |
| Application: |
IHC(paraffin): 10 ug/ml
Western blot analysis: 1-3 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
HeLa cell lysate |
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Background
HAPLN3 is a 360 amino acid extracellular protein belonging to the HAPLN family and contains one Ig-like V-type (immunoglobulin-like) domain and two Link domains. This protein may function in hyaluronic acid binding and cell adhesion. It is either a secreted or an extracellular matrix protein involved in stabilizing the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix. HAPLN3 is widely expressed in most tissues with highest levels in spleen and placenta. |
Antigen
A portion of amino acids 1-50 of human HAPLN3 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a and CRA_b) and chimpanzee and 95% homologous in monkey, 78% homologous in dog and 76% homologous in cow. |
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Western blot analysis of human HAPLN3 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using IMG-6057A at 2 ug/ml. |
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IHC analysis of human placenta using IMG-6057A at 10 ug/ml. |
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Reference
1. Spicer, AP. et al. J. Biol. Chem. 278:21083-21091 (2003).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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