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Polyclonal Antibody to Wnt5A |
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| Catalog No: |
IMG-6075A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein G chromatography |
| Species: |
Cow, Human, Mouse, New World monkey, Rat |
| Predicted React: |
Chimpanzee, Opossum, Pufferfish, Xenopus |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5-10 ug/ml
Western blot analysis: 0.5-2 ug/ml |
| Storage: |
Store Wnt5A antibody at 4°C, stable for 6 months. For long term storage, aliquot and store antibody at -20°C. |
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Background
Wnt5A is a secreted protein found in the extracellular region that belongs to the Wnt family. It is a proto-oncogene that functions in modulating cell fate and behavior during vertebrate development and has an important role in Wnt signaling pathway. It is a glycoprotein that plays an important role in cell communication and development. Wnt5A acts as a ligand for members of the frizzled family of seven transmembrane receptors. It is suspected that Wnt5A is a developmental protein. Wnt5A is a signaling molecule which affects the development of discrete regions of tissues and is likely to signal over only few cell diameters. |
Antigen
A synthetic peptide corresponding to amino acids 195-211 (EFVDARERERIHAKGSY) of human Wnt5A was used as the immunogen for this antibody. |
Application Notes
The amino acid sequence used as immunogen for the Wnt5A antibody is 100% homologous in human, cow, monkey, rat and mouse, 94% in opossum, 88% in xenopus, 81% in chimpanzee and 76% homologous in pufferfish. |
Genebank Info (Protein)
NP_003383 |
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Western blot analysis of Wnt5A in human heart lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat heart using Wnt5A antibody at 0.5 ug/ml. |
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Immunohistochemical analysis of Wnt5A in formalin-fixed, paraffin-embedded human breast tumor using an isotype control (left) and Wnt5A antibody (right) at 10 ug/ml. |
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Reference
1. Clark, CC. et al. Genomics. 18:249-260 (1993).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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