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Product : Antibody >> Apoptosis , New Antibodies >> APG5L
      
 

Peptide-affinity Purified Polyclonal Antibody to APG5L

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6085A
APG5LPurified, Peptide Affinity
0.1 mg
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Catalog No: IMG-6085A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Purification: Peptide affinity chromatography
Species: Bovine, Human, Mouse, Pig, Rat
Predicted React: Pufferfish, Zebrafish
Host: Rabbit
Application: IHC(paraffin): 5 ug/ml
Western blot analysis: 0.5-2 ug/ml
Storage: Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.
Recommended
Positive Control:
T98G cell lysate
 

Background
ATG5L is a 275 amino acid protein belonging to the ATG5 family and is required for autophagy. It plays an important role in the apoptotic process, within the modified cytoskeleton. It is known to function in autophagy, contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. Down-regulation of its expression in HeLa cells suppresses cell death and vacuole formation induced by IFN-gamma. It is highly expressed in apoptotic cells.

Antigen
A portion of amino acid 1-50 of human APG5L was used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, pig and bovine, 87% homologous in pufferfish and 81% homologous in zebrafish.

Genebank Info (Protein): NP_004840
Gene ID: 9474
 
APG5L Antibody

Western blot analysis of human APGL5 in T98G cell lysate in the 1) absence, 2) presence of immunizing peptide using IMG-6085A at 1ug/ml.

APG5L Antibody IMG-6085A

IHC analysis of human kidney using IMG-6085A at 5 ug/ml.

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Reference
1. Pyo, JO. et al.  J. Biol. Chem. 280:20722-20729 (2005).
2. Tanida, I. et al. J. Biol. Chem. 277:13739-13744 (2002).
3. Yung, HW. et al.  FEBS. Lett. 531:168-172 (2002).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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