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Product : Antibody >> Innate Immunology , New Antibodies , Th17 Antibodies >> EBI3
      
 

Polyclonal Antibody to human EBI3

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6100A
EBI3Purified
0.1 mg
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Catalog No: IMG-6100A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05%BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein G Chromatography
Species: Chimpanzee, Human
Predicted React: New World monkey
Host: Rabbit
Application: Western blot analysis: 2-5 ug/ml
Storage: Store at 4C, stable for 6 months. For long term storage, aliquot and store at -20C.
 

Background
EBI3 is a 229 amino acid cytokine belonging to the type I cytokine receptor family (type 3 subfamily) with a signal peptide, 2 potential N-linked glycosylation sites, an LSDWS motif, 2 fibronectin type-III domains. It is usually induced due to infection of B lymphocytes by Epstein-Barr virus and due to expression of an EBV encoded latent membrane protein. EBI3 is a subunit of two heterodimeric cytokines interleukin-27 (IL27) and IL35. It is a secreted glycoprotein associated with calnexin and is a heterodimer with an alpha and a beta subunit. It is also known to interact with SQSTM1 and is responsible for the rapid clonal expansion of naive but not memory CD4 (+) T-cells. It strongly synergizes with IL-12 and trigger IFN-gamma production of naive CD4 (+) T-cells. It mediates its biologic effects through the cytokine receptor WSX-1/TCCR. It triggers signaling in T cells, B cells, and myeloid cells and along with p35 it acts as an important immunomodulator. It is usually expressed in various hemopoietic tissues and cell lines.

Antigen
A portion of amino acids 120-170 of human EBI3 was used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in human (isoform CRA_a and CRA_b), chimpanzee and 94% homologous in monkey.

EBI3 is routinely seen in western blot at approximately 35 kD.

View additional EBI3 antibodies

Genebank Info (Protein): NP_005746
Gene ID: 10148
SwissProt: Q14213
 
EBI3 Antibody

Western blot analysis of EBI3 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide at 2 ug/ml and 3) recombinant human IL-35 protein at 5 ug/ml using EBI3 antibody.

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Reference
1. Collison, LW. et al. Nature. 450:566-569 (2007).
2. Pflanz, S. et al. J. Immun. 172:2225-2231 (2004).
3. Pflanz, S. et al. Immunity. 16:779-790 (2002).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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