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Polyclonal Antibody to HDAC-7 (Phospho-Ser155) |
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| Catalog No: |
IMG-6125A |
| Contents: |
50 ug in 100 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Chimpanzee, Cow, Dog, Human, New World monkey, Mouse, Rat |
| Predicted React: |
Opossum, Zebrafish |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 0.1-1 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C |
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Background
Histone deacetylase 7 is a thymus-specific, intracellular transcription factor belonging to the class IIa histone deacetylase family. Reports suggest that dephosphorylation and nuclear localization of HDAC-7 is promoted by myosin phosphatase, thus regulating the repression of Nur77 and inhibition of apoptosis in CD4+CD8+ double-positive thymocytes. HDAC-7 plays a specific role in maintaining vascular integrity by repressing the expression of matrix metalloproteinase (MMP) 10. It promotes repression mediated by transcriptional corepressor NCOR2 and is an efficient corepressor of the androgen receptor (AR). It is also responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). |
Antigen
A synthetic peptide containing phospho-serine 155 of human HDAC-7 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, mouse, monkey, dog, chimpanzee, rat and cow, 90% homologous in opossum and zebrafish. |
| Genebank Info (Protein): NP_055522 |
| Gene ID: 9734 |
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Western blot analysis of phosphorylated HDAC-7 in (A) recombinant fusion protein containing a phosphorylated serine at position 155 and (B) fusion protein containing an unphosphorylated serine at position 155, using IMG-6125A at 0.1 ug/ml. |
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Reference
1. Maribel, P. et al. GENES & DEVELOPMENT. 21:638-643 (2007).
2. Chang, S. et al. Cell. 126:321-334 (2006).
3. Dequiedt, F. et al. Immunity. 18:687-698 (2003).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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