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Polyclonal Antibody to Bnc 1 |
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| Catalog No: |
IMG-6130A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein G Chromatography |
| Species: |
Chimpanzee, Human, New World monkey |
| Predicted React: |
Mouse, Rat, Horse, Dog, Cow |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 4-7 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
Human and rat spleen lysate |
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Background
Bnc 1 is a 994 amino acid transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. It is a zinc finger protein with three separated pairs of zinc fingers and a nuclear localization signal. Bnc 1 is a soluble protein that can shuttle between the nucleus and the cytoplasm, and its location depends on the proliferative potential of the cell. It is expressed relatively uniformly in the nucleoplasm, and phosphorylation on Ser-537 and Ser-541 leads to its cytoplasmic localization. It is present in the basal cell layer of the epidermis, in hair follicles and also in abundance in the germ cells of testis and ovary, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart. Reports suggest that it plays a regulatory role in keratinocyte proliferation and also in rRNA transcription. It is also known to play a role in the differentiation of spermatozoa and oocytes. |
Antigen
A portion of amino acids 330-380 of human Bnc 1 protein was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, monkey and chimpanzee, 92% homologous in cow and horse, 85% homologous in mouse and dog, and 78% homologous in rat. |
Genebank Info (Protein)
NP_001708 |
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Western blot analysis of Bnc 1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, and 3) rat lysate using IMG-6130A at 7 ug/ml and 4 ug/ml, respectively. |
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Reference
1. Vanhoutteghem, A. et al. Proc. Natl. Acad. Sci. USA. 103:12423-12428 (2006).
2. Tseng, H. et al. J. Cell. Sci. 18:3039-3047 (1999).
3. Tseng, H. et al. Proc. Nat. Acad. Sci. 89:10311-10315 (1992).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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