PATHWAY PROFILING ANTIBODIES & REAGENTS

     
Products
Product : Antibody >> Chromatin , New Antibodies >> Bnc 1
      
 

Polyclonal Antibody to Bnc 1

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6130A
Bnc 1Purified
0.1 mg
 --
 
Catalog No: IMG-6130A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein G Chromatography
Species: Chimpanzee, Human, New World monkey
Predicted React: Mouse, Rat, Horse, Dog, Cow
Host: Rabbit
Application: Western blot analysis: 4-7 ug/ml
Storage: Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.
Recommended
Positive Control:
Human and rat spleen lysate
 

Background
Bnc 1 is a 994 amino acid transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. It is a zinc finger protein with three separated pairs of zinc fingers and a nuclear localization signal. Bnc 1 is a soluble protein that can shuttle between the nucleus and the cytoplasm, and its location depends on the proliferative potential of the cell. It is expressed relatively uniformly in the nucleoplasm, and phosphorylation on Ser-537 and Ser-541 leads to its cytoplasmic localization. It is present in the basal cell layer of the epidermis, in hair follicles and also in abundance in the germ cells of testis and ovary, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart. Reports suggest that it plays a regulatory role in keratinocyte proliferation and also in rRNA transcription.  It is also known to play a role in the differentiation of spermatozoa and oocytes.

Antigen
A portion of amino acids 330-380 of human Bnc 1 protein was used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, monkey and chimpanzee, 92% homologous in cow and horse, 85% homologous in mouse and dog, and 78% homologous in rat.

Genebank Info (Protein): NP_001708
Gene ID: 646
SwissProt: Q01954
 
Bnc 1 Antibody

Western blot analysis of Bnc 1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, and 3) rat lysate using IMG-6130A at 7 ug/ml and 4 ug/ml, respectively.

-

Reference
1. Vanhoutteghem, A. et al. Proc. Natl. Acad. Sci. USA. 103:12423-12428 (2006).
2. Tseng, H. et al. J. Cell. Sci. 18:3039-3047 (1999).
3. Tseng, H. et al. Proc. Nat. Acad. Sci. 89:10311-10315 (1992).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


Products

Services

Resources

Purchasing

Company

  • What's New
  • Reporter Cells
  • Screening Services
  • Interactive Pathways