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Product : Antibody >> Chromatin , DNA Methylation & Rep.. , New Antibodies >> DMAP1
      
 

Polyclonal Antibody to DMAP1

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6142A
DMAP1Purified
0.1 mg
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Catalog No: IMG-6142A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein G Chromatography
Species: Chimpanzee, Cow, Dog, Human, Mouse, Rat
Predicted React: Chicken, Zebrafish
Host: Rabbit
Application: IHC (paraffin): 5 ug/ml
Western blot analysis: 1.0-4.0 ug/ml
Storage: Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.
Recommended
Positive Control:
Human, mouse and rat brain lysate
 

Background
DNA methyltransferase 1-associated protein 1 (DMAP1) is involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The protein also specifically potentiates DAXX-mediated repression of glucocorticoid receptor-dependent transcription. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. DMAP1 is found in a wide variety of living organisms from yeasts to humans. The human DMAP1 gene is localized on chromosome 1p34.

Antigen
A portion of amino acids 250-300 of human DMAP1 was used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in chimpanzee, dog, human, rat, cow, mouse, 88% homologous in chicken and 77% homologous in zebrafish. The approximately 27 kD band seen in brain lysate is also seen in the prebleed and not thought to be relevant.

Genebank Info (Protein): NP_001029196
Gene ID: 55929
SwissProt: Q9NPF5
 
DMAP1 Antibody

Western blot analysis of DMAP1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain using IMG-6142A at 4.0 ug/ml, 1.0 ug/ml and 1.0 ug/ml, respectively.

DMAP1 Antibody IMG-6142A

Immunohistochemical analysis of DMAP1 in paraffin-embedded formalin-fixed mouse brain tissue using an isotype control (top) and IMG-6142A (bottom) at 5 ug/ml.

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Reference
1. Liu, Z. et al. J. Biol. Chem. 279:14120-14128 (2004).
2. Muromoto, RJ. et al. Immunol. 172:2985-2993 (2004).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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