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Polyclonal Antibody to DMAP1 |
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| Catalog No: |
IMG-6142A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein G Chromatography |
| Species: |
Chimpanzee, Cow, Dog, Human, Mouse, Rat |
| Predicted React: |
Chicken, Zebrafish |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5 ug/ml
Western blot analysis: 1.0-4.0 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
Human, mouse and rat brain lysate |
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Background
DNA methyltransferase 1-associated protein 1 (DMAP1) is involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The protein also specifically potentiates DAXX-mediated repression of glucocorticoid receptor-dependent transcription. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. DMAP1 is found in a wide variety of living organisms from yeasts to humans. The human DMAP1 gene is localized on chromosome 1p34. |
Antigen
A portion of amino acids 250-300 of human DMAP1 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in chimpanzee, dog, human, rat, cow, mouse, 88% homologous in chicken and 77% homologous in zebrafish.
The approximately 27 kD band seen in brain lysate is also seen in the prebleed and not thought to be relevant. |
Genebank Info (Protein)
NP_001029196 |
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Western blot analysis of DMAP1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain using IMG-6142A at 4.0 ug/ml, 1.0 ug/ml and 1.0 ug/ml, respectively. |
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Immunohistochemical analysis of DMAP1 in paraffin-embedded formalin-fixed mouse brain tissue using an isotype control (top) and IMG-6142A (bottom) at 5 ug/ml. |
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Reference
1. Liu, Z. et al. J. Biol. Chem. 279:14120-14128 (2004).
2. Muromoto, RJ. et al. Immunol. 172:2985-2993 (2004).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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