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Polyclonal Antibody to APG16L |
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| Catalog No: |
IMG-6163A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein G Chromatography |
| Species: |
Chimpanzee, Human, New World monkey, Mouse |
| Predicted React: |
Chicken, Dog |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 1.0-3.0 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
Human skeletal muscle lysate |
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Background
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes/vacuoles. The process is found in eukaryotes, including yeast, and involves several genes and two novel ubiquitin-like conjugation systems. The APG12 system is one of the ubiquitin-like protein conjugation systems essential for autophagy. Covalent attachment of APG12–APG5 is essential for autophagy. In mammalian cells, the APG12–APG5 conjugate is a protein complex of approximately 800 kDa and includes an additional novel protein. This novel protein was designated APG16L (APG16-like, also referred to as ATG16L) as it's N-terminal region contains several features similar to yeast APG16. APG16L protein has several spliced isoforms and is larger than yeast APG16 (17 kD). The protein has a large C-terminal domain containing seven WD repeats. Genetic variations in the protein are associated with susceptibility to inflammatory bowel disease type 10. The human APG16L gene is localized in the chromosomal region 2q37.1. |
Antigen
A portion of amino acids 25-75 of human APG16L was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, chimpanzee and monkey, 94% homologous in dog and mouse and 77% homologous in chicken.
Different species have multiple isoforms of APG16L. Human APG16L has at least six isoforms: CRA_a at 607 amino acids, CRA_b at 588 amino acids, CRA_c at 309 amino acids, CRA_d at 374 amino acids, CRA_e at 284 amino acids and CRA_f at 463 amino acids. Multiple isoforms of APG16L can also be detected in mouse skeletal muscle lysate using IMG-6163A.
The approximately 40 kD band seen in skeletal muscle lysate is also seen in prebleed and is not believed to be relevant. |
Genebank Info (Protein)
NP_110430 |
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Western blot analysis of APG16L in human skeletal muscle lysate in the 1) presence and 2) absence of immunizing peptide using IMG-6163A
at 2.0 ug/ml. |
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Reference
1. Mizushima, N. et al. Nature. 395:395-398 (1998).
2. Mizushima, N. et al. The International Journal of Biochemistry & Cell Biology. 35:553-561 (2003).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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