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Product : Antibody >> Apoptosis , Autophagy , Cancer Antibodies , Caspases , CD Markers >> Western Blot Loading Control Kit
      
 

Western Blot Loading ControláSampler Kit

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6166K
Western Blot Loading Control KitKit
0.05 mg each
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Catalog No: IMG-6166K
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions ľ novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: Primay antibodies contain 50 ug in 100 ul PBS with 0.05% BSA and 0.05% sodium azide. Secondary antibody contains 50 ul antibody in PBS and 0.05% BSA, no azide.á BSA is of U.S. origin.
Isotype: Rabbit IgG
Clone: N/A
Purification: Protein G Chromatography
Species: Multi
Host: Rabbit
Application: Western blot analysis: Please see individual data sheets for details.
Storage: Store at 4░C, stable for 6 months. For long-term storage, store at -20░C.
Recommended
Positive Control:
Please see individual data sheets for details.
 

Background

Genes that are constitutively and stably expressed at high levels in almost all tissues and cells are often referred to as housekeeping genes. Housekeeping proteins such as GAPDH, beta Actin, beta Tubulin, or Histone H2B (nuclear fractions) are useful as loading controls for western blots and protein normalization. Increasingly, researchers are making use of specific antibodies to housekeeping proteins to standardize and compare levels of protein. However, studies examining gene transcript and protein levels have shown that expression levels of housekeeping genes can vary. Collectively the results indicate that some housekeeping proteins may be better suited than others for use as loading controls depending on the model system used.

The Western Blot Loading Control Sampler Kit is a cost-effective tool containing a panel of four antibodies specific to proteins frequently used for protein normalization, plus appropriate secondary reagent. Sufficient quantity of each antibody is included for several mini-blots; see individual datasheets for additional information on species reactivity and suggested dilutions. 

  Description Cat. No. Qty Appl Reactivity Format
  beta Actin IMG-5142A 50 ug WB H, M, R Purified
  beta Tubulin IMG-5810A 50 ug WB Bo, Ch, D, H, M, Mk, R, Xe Purified
  GAPDH   IMG-5143A 50 ug WB Ca, Chp, Dros, H, M, Mq, Pi, R Purified
  Histone H2B IMG-359 50 ug WB Ch, D, Dros, H, M, Ma, R, Zf Purified
  Anti-Rabbit Ig HRP    Secondary 20301 50 ul WB Ra Purified
Bo: Bovine  Ca:Cat  Ch:Chicken  Chp:Chimpanzee  D:Dog  Dros:Drosophila  H:Human  M:Mouse  Ma:Mammals Mk:Monkey  Mq:Macaque  R:Rat  Xe:Xenopus  Zf:Zebrafish

 
Western Blot Loading ControláKit Antibody

Western blot analysis of betaáactin in A: human brain (40141),áB: mouse brain (40121), C: rat brain (40121), D: human lung (40146) and E: human spleen (40149)átissue lysates using IMG-5142A at 0.25 ug/ml.

Western Blot Loading ControláKit Antibody IMG-6166K

Western blot analysis of beta tubulin in A: human brain (40141),áB: mouse brain (40121) and C: rat brain (40121) tissue lysates using IMG-5810A at 0.25 ug/ml.

Western Blot Loading ControláKit

Western blot analysis of GAPDH ináthe multiple human tumor cell lineálysate INSTA-Blotá(IMB-105)áusing IMG-5143A at 0.25 ug/ml.

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Reference
1. Housekeeping genes as internal standards: Use and limits. Thellin O, W Zorzi, B Lakaye, B De Borman, B Coumans, G Hennen, T Grisar, A Igout, and E Heinen. J Biotechnology 75:291-295 (1999).
2. Housekeeping proteins: A preliminary study illustrating some limitations as useful references in protein expression studies. Ferguson RE, HP Carroll, A Harris, EM Maher, PJ Selby, and RE Banks. Proteomics 5:566-571 (2005).
3. Western blotting. Kurien, BT and RH Scofield. Methods 38:283-293 (2006).
4. GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Barber RD, DW Harmer, RA Coleman and BJ Clark. Physiol Genomics 21:389-395 (2005).
5. An error model for protein quantification. Kreutz C, MM Bartolome Rodriquez, T Maiwald, M Seidi, HE Blum, L Mohr, and J Timmer. Bioinformatics 23:2747-2753 (2007).
6. De-regulation of common housekeeping genes in heptocellular carcinoma. Waxman S and E Wurmbach. BMC Genomics doi:10.1186/1471-2164-8-243 (2007).


Research purposes only. Not for diagnostic or in vivo use.


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