|
|
|
| |
Polyclonal Antibody to APG10L |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-6174A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5 ug/ml
Western blot analysis: 2.0-6.0 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
Brain |
|
 |
|
|
|
|
|
|
Background
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes/vacuoles. The formation of autophagosomes involves a dynamic rearrangement of the membrane for which two ubiquitin-like modifications (the conjugation of APG12p and the modification of a soluble form of MAP-LC3 to a membrane-bound form) are essential. APG10L is an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation, ATG12 -ATG5 conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A), a homolog of yeast Apg8, to a membrane-bound form. The protein is ubiquitously expressed. Human APG10L gene is localized on chromosome 5q14.2. |
Antigen
A portion of amino acids 1-50 of human APG10L was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human (isoform 2) and 87% homologous in mouse. |
Genebank Info (Protein)
NP_001124500 |
| |
|
Western blot analysis of APG10L in human brain lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse brain and 4) rat brain using IMG-6174A at 2.0 ug/ml, 6.0 ug/ml and 4.0 ug/ml, respectively. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
|
|
|
|
Immunohistochemical analysis of APG10L in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and IMG-6174A (bottom left, right) at 5 ug/ml. |
|
|
|
|
|
| - |
 |
|
Reference
1. Nemoto, T. et al. J. Biol. Chem. 278:39517-39526 (2003).
2. Baehrecke, EH. et al. Nat. Rev. Mol. Cell. Biol. 6:505-510 (2005).
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
